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impact of elemental sulphur on solubility of soil heavy metals and their uptake by way of maize
pH can also be accomplished by way of application of mineral or biological
acids or acid-producing fertilizers such as ammonium chlo-
experience (Salt et al., 1995; Huang et al., 1997; Wasay et al.,
1998). although, these strategies have some boundaries due
to terrible effects on the chemical, physical and organic
homes of the soil, or may cause groundwater pollutants
through leaching. To evade some of those constraints, the
use of elemental S to decrease soil pH and raise the
solubility of heavy metals in soils has been recommended (Tichy
et al., 1997; Seidel et al., 1998; Kayser et al., 2000).
however, extra investigations are mandatory to evaluate the
feasibility of this system as a tool for the enhancement of
metal solubility and uptake via a metal accumulator, espe-
cially in alkaline or neutral soils contaminated through distinct
concentrations of heavy metals. therefore, the pursuits of
this examine were to (1) examine the metallic solubility of soil
with diverse concentrations of Pb, Zn and Cd addition via
software of S and (2) examine the knowledge to enhance
steel uptake and accumulation via maize via applica-
tion of S.
2. substances and methods
2.1. Soil features
Soil (clay loam) became accumulated from the appropriate 20 cm of
the profile in Qinghe, Beijing, China. The physicochem-
ical houses of the soil before the test have been: pH
7.7, biological mater content 22 g kg
, total Pb one hundred thirty mg
, complete Zn 120 mg kg
, total Cd 0.05 mg kg
-extractable Pb 0.forty five mg kg
0.ninety eight mg kg
2.2. Pl ants and cultivation
Maize (Zea maysL.) seeds were sown at a fee of 4
and thinned to leave two seedlings in each pot 1 week
after germination. The test was performed in a
greenhouse with a 14 h (26 jC)/10 h (13 jC) day/nighttime
cycle. Soil moisture content material changed into adjusted always by means of
weight to about 60% of water conserving ability and the
plants were grown for forty days.
2.three. Experimental design and treatments
The experimental designs had been employed with elemental
sulphur (S) and Pb, Zn or Cd. Sulphur turned into applied and
blended absolutely with the soil at two prices, 0 (S
), and 200
) mmol kg
soil and three heavy metals utilized with
three fees, Pb at 0 (Pb
), 200 (Pb
), four hundred (Pb
soil, Zn at 0 (Zn
), one hundred (Zn
), 200 (Zn
and Cd at 0 (Cd
), 50 (Cd
), one hundred (Cd
respectively. there were 18 cures in total and each one
with three replicates. The particular person metals were added by way of
metal solutions (Pb(NO
changed into brought, the adding concentrations of the three metal
options had been 38.6, sixty one.2 and 17.8 mmol l
Fertilizer turned into utilized at the prices of one hundred twenty mg N (as
), 35 mg P (as KH
) and a hundred mg okay (as KCl
soil earlier than seeding, which become about 12
days after metal contaminants have been added in. every pot
bought 1 kg of soil that become allowed to equil ibrate for a
duration of 14 days after S was put in then the plant seeds
had been sown in the greenhouse.
2.4. Sampling and analysis
The soil samp les had been taken earlier than and after the exper-
iment, then air dried, floor and sieved passing through a
2-mm sieve. Soil pH become measured with subsamples the use of a
1:2.5 (soil/0. 01 M CaCl
) ratio by a pH meter. For soil
extractable metals, 20 ml of 0.01 M CaCl
have been added to
2.00 g of air-dried soil in a 50-ml polypropylene centrifuge
tube. The tube changed into shaken conclusion-over-conclusion for 16 h at 25 jC,
centrifuged at 4000 rpm for 10 min. The supernatant turned into
decanted and saved for Pb, Zn and Cd choice via
atomic absorption spectrophotometry (AAS: Z-6100 Hita-
chi, Japan) and inductively coupled plasma-optical emission
spectroscopy (ICP-OES: Optima-2000, Perkin-Elmer, us of a)
in an effort to make certain the outcomes are similar (Novozamsky et
al., 1993; Degryse et al., 2003). For the full volume of
metals, the air-dried soil turned into floor and sieved via passing
through a 0.25-mm sieve and the subsamples had been digested
with a mixture of targeted HNO
(the concentrations of HNO
and HF had been 15.6,
11.7 and 19.7 mol l
, respectively) after which the overall
concentrations of Pb, Zn and Cd had been determined by using AAS.
After plant harvest, the soil samples have been taken, air dried,
floor and sieved by passing through a 2-mm sieve. Soil
-extractable Pb, Zn and Cd have been also decided
with the identical methods as described above.
all the plant life have been harvested after the test and the
plant samples were rinsed with deionized water, oven dried
at 70 jC for 48 h, ground with an agate mill, digested with a
combination of targeted HNO
/HF (three:1:1 in vol-
ume), and analyzed by means of AAS. The records have been subjected to
ANOVA and subsequently to Duncan’s distinct latitude test
by using software of SAS kit (edition eight.1, SAS, Cary, NC,
three. effects and dialogue
three.1. Soil pH
Soil pH reduced in the entire remedies with utility
of S compared to the control (table 1). One important reality is
the oxidation of S with the aid of certain agencies of acidop hilic bacteria,
specially Thiobacillusspp. within the soil (Lee et al., 1988; Seidel
et al., 1998; Tichy
et al., 1997; Kayser et al., 2000).Tichy
al. (1997) said that 156 mmol of several types of
Y. Cui et al. / ambiance foreign 30 (2004) 323–328324