|Exam Name||:||Certified Internet Associate (JNCIA-SSL) 562|
|Questions and Answers||:||240 Q & A|
|Updated On||:||April 18, 2019|
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Juniper Networks helps organizations build excessive-efficiency networks by means of presenting networking gadget, features and its own community operating device, Junos. With about 9,800 personnel global, Juniper Networks gives networking functions to the realm's desirable one hundred provider suppliers as well as banks, stock exchanges, government groups, fitness care corporations and other industries.
To aid its big selection of networking applied sciences and the customers who use them, the Juniper Networks Certification application offers a couple of credentials at the affiliate, expert, knowledgeable and professional degrees.
A Juniper Networks certification can distinguish you from noncertified peers, give career boom opportunities, provide you with enhanced insight into Juniper Networks' products and capabilities and improvement your purchasers. as a consequence, those that work – or aspire to work – in Juniper-primarily based networking environments will locate their certifications positive for career building and enhancement.Juniper Networks Certification program Overview
Juniper Networks' Certification application (JNCP) has a couple of tracks, categorized by way of career route. Its credentials are in response to Juniper's technologies, items and capabilities. JNCP presents seven tracks together with:
We check with these credentials collectively as "network engineer" certifications as a result of they are all geared towards community engineering gurus. each song is coated in more detail in right here sections. which you could additionally discover more particulars on the Juniper Networks Certification Tracks pages.
every certification requires one exam, together with relevant necessities. affiliate-stage certification tests can charge $200, specialist checks are $300 and skilled exams are $four hundred, all of which are provided through Pearson VUE. knowledgeable-degree certifications require a fingers-on lab examination, which charges $1,400 and is purchasable simplest at select Juniper community testing facilities.
Juniper Networks requires certification holders to recertify each three years to retain their credentials. For affiliate, professional and professional certifications, credential holders have to pass the current examination. in contrast to different IT certification courses, however, passing an exam (or taking an authorised path) additionally renews all decrease-stage certifications executed on the equal music. to resume an expert-level certification, candidates should pass the existing professional-level examination.JNCIA-Junos Certification
The Juniper Networks certified associate - Junos (JNCIA-Junos) is the entry-level certification for all of the company's certification tracks except the Cloud and Design tracks. The Cloud certification course takes the JNCIA-Cloud credential whereas the Design certification path takes the Juniper Networks licensed Design affiliate (JNCDA) as its entry-level credential
The JNCIA-Junos credential is geared towards people who have basic experience with the Juniper Networks Junos OS, and consider the basics of networking, routing and switching. You have to achieve this certification (or the JNCIA-Cloud for the Cloud course and JNCDA for the Design direction) to advance to the expert, professional and skilled certs. To earn the JNCIA-Junos, candidates deserve to pass the JN0-102 exam which has 65 distinct-alternative questions and takes up to ninety minutes to finished.JNCP enterprise Routing and Switching tune
The JNCP commercial enterprise Routing and Switching tune is effective for people educated in networking technologies in established and Juniper Networks' business routing and switching structures particularly. The four certifications during this tune kind a certification ladder, with each and every certification acting as a prerequisite to the subsequent cert in the sequence:
starting at the backside of the ladder, the JNCIA-Junos certification is a prerequisite to the JNCIS-ENT certification.
The JNCIS-ENT acknowledges networking professionals with fundamental to intermediate knowledge of routing and switching, platform configuration and troubleshooting in Junos. Candidates should pass a single written exam (JN0-347), which incorporates sixty five multiple-option questions and takes as much as ninety minutes to comprehensive.
JNCIS-ENT is a prerequisite to the professional-degree JNCIP-ENT certification, which is pertinent to networking specialists who've extensive competencies of enterprise routing and switching applied sciences, platform configuration and Junos OS troubleshooting talents. Candidates take examination JN0-647 which has sixty five numerous-option questions and takes as much as 120 minutes to comprehensive.
The JNCIP-ENT is a prerequisite to the knowledgeable-stage JNCIE-ENT certification, which is the optimum certification in the commercial enterprise Routing and Switching tune. This certification requires a practical examination that assessments the networking knowledgeable's means to installation, configure, control and troubleshoot Junos-primarily based enterprise routing and switching systems using loads of contraptions, protocols and features. Candidates can join a JNCIE bootcamp to put together for the JPR-943 functional examination, which lasts as much as eight hours.Junos protection song
Junos' safety certifications encompass affiliate, expert, skilled and knowledgeable stages. the 1st step is the associate-stage JNCIA-Junos certification. or not it's a prerequisite to the next certification, the Juniper Networks certified specialist safety (JNCIS-SEC). This intermediate-stage certification is applicable to candidates who configure and troubleshoot Junos' software for SRX collection gadgets and have in mind safety applied sciences. The JN0-333 written examination takes as much as ninety minutes and consists of sixty five questions.
JNCIS-SEC is a prerequisite to the Juniper Networks certified expert security (JNCIP-SEC) credential. It recognizes networking gurus who're highly accepted with safety, platform configuration and troubleshooting of the Junos utility for SRX series gadgets. The JN0-634 written examination has 65 assorted-option questions and takes as much as one hundred twenty minutes.
JNCIP-SEC is a prerequisite to the Juniper Networks certified knowledgeable safety (JNCIE-SEC), essentially the most advanced certification in the protection track. The JNCIE-SEC identifies authorities who put in force, configure, manage and troubleshoot Junos-primarily based security platforms. The JPR-932 practical exam lasts for as much as eight hours.Junos carrier company Routing And Switching song
Like other Juniper Networks certification tracks, the service issuer Routing and Switching tune has associate, professional, expert and expert certifications. JNCIA-Junos is the foundational certification from which a candidate can achieve the Juniper Networks licensed information superhighway expert (JNCIS-SP), then Juniper Networks' certified information superhighway knowledgeable (JNCIP-SP) and the Juniper Networks licensed information superhighway expert (JNCIE-SP) credentials.
where the business Routing and Switching music focuses specially on statistics core design principles, in addition to firewalls and VPNs, the carrier issuer Routing and Switching tune zeroes in on WAN design and Juniper Networks' QFabric for Layer 2/Layer 3 switching.
JNCIS-SP identifies individuals with newbie to intermediate skills of routing and switching implementations in Junos. The JN0-361 written exam takes up to 90 minutes to comprehensive and has sixty five questions.
JNCIP-SP applies to experienced networking gurus with superior talents of routing technologies and platform configuration and who can troubleshoot Junos OS. Juniper introduced a brand new JNCIP-SP exam, the JNO-662 examination, on April 1, 2018. Candidates may also nonetheless examine on the ancient JNO-661 examination except may 15, 2018. The JN0-661 and JNO-662 written exams take up to a hundred and twenty minutes and have sixty five questions.
JNCIE-SP is the top certification in this music. It requires passing an eight-hour purposeful examination (NPR-960) for networkers who put in force, troubleshoot and hold Juniper Networks service company networks.Junos data middle tune
one of the vital newer additions to the Juniper Networks Certification program is the data core certification music. The statistics core track is designed for experts who deploy, configure and manage records middle technologies and the Junos OS in addition to troubleshoot issues.
Candidates may still begin by means of earning the JNCIA-Junos credential which serves as a prereq to the professional-degree JNCIS-DC certification. An intermediate credential, the JNCIS-DC validates a candidate’s competencies of routing and switching, troubleshooting, and platform configurations. To earn the JNCIS-DC credential, candidates ought to flow the JNO-347 exam which takes ninety minutes and has sixty five questions.
From the JNCIS-DC cert, candidate’s will growth to the JNCIP-records center (JNCIP-DC) cert. knowledgeable cert for advanced practitioners, the JNCIP-DC takes the JNCIS-DC as a prerequisite, and requires candidates to move one exam. The JN0-680 exam has 65 questions and takes as much as a hundred and twenty minutes to comprehensive. Candidates are expected to be aware chassis fabric (actual and digital), IP cloth, statistics core interconnects and more.
on the professional level is the JNCIE-DC, which acknowledges authorities who construct, configure and manage data core networks that consist of Juniper Networks' MX series and QFX series devices. They ought to additionally wholly be aware several other applied sciences, such as Clos IP cloth, Ethernet VPNs, digital extensible VLANs and sophistication of service (CoS) points. Like other professional certifications, candidates must flow an eight-hour lab examination.JNCP Automation and DevOps tune
The Juniper Networks Automation and DevOps certification music validates a candidate’s knowledge of scripting languages, automation of Junos-linked networks or gadgets, as neatly as the capability to apply automation to Junos methods.
As with different Juniper certification tracks, candidates begin via acquiring the JNCIA-Junos affiliate credential which serves as a prereq to the expert level JNCIS-DevOps certification. The JNCIS-DevOps objectives advantage of scripting and automation tools together with Phython, Ansible, and PyEZ, utility of tools to Junos networks and instruments, and top-rated practices. To earn the credential, candidates need to move JNO-420 exam which has 65 questions and takes 90 minutes to complete.
at present, these are the only certifications within the Automation and DevOps song.JNCP Cloud track
experts fascinated in cloud-based networks should comply with the Juniper Cloud certification track. Two Cloud credentials are at present available – one on the associate and the different on the expert degree. Cloud certifications validates candidates talents of cloud-primarily based networking concepts, architectures, applied sciences, and conception.
Candidates interested in the Cloud certification track start their event with the associate-level JNCIA-Cloud credential. To earn this credential, candidates must take the JNO-210 examination which has sixty five questions and lasts up to ninety minutes. The JNCIA-Cloud certification serves as a prerequisite for the expert-level JNCIS-Cloud certification. JNCIS-Cloud candidates should still possess an figuring out of SD-WAN, multi-cloud and different cloud networking architectures along with the potential to install and configure Juniper cloud networks. To earn the credential, candidates take the JNO-411 exam which has sixty five questions and takes 90 minutes to finished.JNCP Design tune
professionals drawn to Juniper community design concepts may still pursue the Juniper Design certification song. The Design track pursuits applied sciences and principles regarding Juniper Networks, including principles of design concept and premiere practices.
Juniper presents Design credentials at the associate and specialist level. The Juniper Networks certified Design affiliate (JNCDA) is the groundwork credential on the Design certification track, validating a candidate’s knowledge of community design fundamentals. Candidates should pass the JNO-1100 examination to earn the credential.
The JNCDA certification serves as a prereq to the specialist-degree credential. There are three answer tracks accessible on the specialist degree:
All exams are sixty five questions and take in to 90 minutes to complete.connected Jobs, practising and materials
Juniper Networks' certifications most frequently lead to community engineer positions, both with Juniper Networks itself, or with consulting establishments that guide the business's products. which you can additionally locate network administrator, community protection engineer and network operations center (NOC) engineer job roles linked to Juniper certs as well as technical income positions.
greater than 2,500 positions bubble up on such job boards at certainly and SimplyHired when attempting to find the keywords "Juniper Networks," a fair number of which require or need a Juniper Networks certification.
in line with SimplyHired, general network engineer salaries start at $78,000 nationwide with earnings on the high aspect topping $a hundred twenty five,000, while Juniper Networks salary have been suggested at $137,000. Getting the necessary event and a couple of Juniper certifications can pay off handsomely. reaching salaries within the greater conclusion of the range typically ability having five to nine years of experience and maybe a bachelor's diploma (or larger).
despite the fact each and every certification tune's internet page lists some examination prep materials, a superb first cease is Juniper's Certification brief hyperlinks. here you are going to find hyperlinks to the a variety of certification tracks, counseled working towards, route materials and more. every certification web page additionally includes links to suggested training substances, practices tests and additional learning elements. another easy aid is Junos Genius, a certification education app with apply questions.
Candidates can take practising courses, boot camps and expertise camps through approved schooling partners worldwide to get palms-on experience with Juniper gadget, which is needed to succeed on certification tests. The Juniper practicing website gives a link to informed classes, on-demand practicing, open studying and boot camps (where relevant and obtainable) in addition to different examination training materials.
Bootcamps are 5-day, intensive, lab-based mostly practicing classes for expert-level checks. Juniper Networks' boot camps typically can charge greater than $four,000, youngsters fees vary reckoning on company and placement. (Some boot camps consist of a complimentary examination voucher.) To participate in a boot camp, you ought to have already earned the prerequisite certifications for whatever thing exam(s) you are making ready to address.
Juniper Networks certified internet affiliate (JNCIA) Median Hourly cost with the aid of Job
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exceptionjava.lang.IllegalStateException: provider did not delivery accurately com.eidosmedia.portal.impl.config.PortalRequestInitializer.requestInitialized(PortalRequestInitializer.java:72) org.apache.catalina.valves.ErrorReportValve.invoke(ErrorReportValve.java:79) org.apache.catalina.valves.AbstractAccessLogValve.invoke(AbstractAccessLogValve.java:620) com.eidosmedia.portal.catalina.CobaltValve._invoke(CobaltValve.java:390) com.eidosmedia.portal.catalina.CobaltValve.invoke(CobaltValve.java:176) org.apache.catalina.valves.RemoteIpValve.invoke(RemoteIpValve.java:676) org.apache.catalina.connector.CoyoteAdapter.service(CoyoteAdapter.java:502) org.apache.coyote.http11.AbstractHttp11Processor.manner(AbstractHttp11Processor.java:1104) org.apache.coyote.AbstractProtocol$AbstractConnectionHandler.system(AbstractProtocol.java:684) org.apache.tomcat.util.net.NioEndpoint$SocketProcessor.doRun(NioEndpoint.java:1519) org.apache.tomcat.util.internet.NioEndpoint$SocketProcessor.run(NioEndpoint.java:1475) java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1142) java.util.concurrent.ThreadPoolExecutor$employee.run(ThreadPoolExecutor.java:617) org.apache.tomcat.util.threads.TaskThread$WrappingRunnable.run(TaskThread.java:61) java.lang.Thread.run(Thread.java:748)
notice the total stack hint of the root trigger is purchasable in the Apache Tomcat/8.0.forty two logs.Apache Tomcat/eight.0.42
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Ads for online schools are all over the Internet, plastered on billboards in subway cars and on television. The University of Phoenix, with nearly 500,000 students, is the biggest for-profit college. But some former students said they were duped into paying big bucks and going deeply in debt by slick and misleading recruiters.
"I don't want anyone else to be sucked in," said Melissa Dalmier, 30, of Noble, Ill.
The mother of three had big dreams to be an elementary school teacher, so when she saw ads for the University of Phoenix pop-up on her computer, she e-mailed them for more information. A few minutes later, Dalmier said she got a call from one of the school's recruiters, who she said told her that enrolling in the associate's degree in education program at the University of Phoenix would put her on the fast-track to reaching her dream.
"[The recruiter said] they had an agreement with Illinois State Board of Education and that as soon as I finished their program I'd be ready to start working," she recalled.
Within 15 minutes, Dalmier was enrolled. Since she didn't have enough money to pay for tuition, she said the recruiter helped her get federal student aid. In total, she took out about $8,000 in federally-guaranteed student loans.
But just a few months after Dalmier started, she said she learned the horrible truth: the degree program she was enrolled in would not qualify her to become a public school teacher upon graduation in Illinois.
"It was an outright lie. A bold faced lie," she said.
It's not the first time that the controversial school, which obtains almost 90 percent of its revenues from students paying tuition from federal aid, has come under fire for its recruiting methods.
The University of Phoenix was one of 15 for-profit schools whose aggressive recruiting practices were the subject of hearings held by Sen. Tom Harkin, D-Iowa. The Government Accountability Office sent investigators to for-profit schools across the country and found that all of them were misleading potential students.
In 2004, the University of Phoenix paid nearly $10 million to the Department of Education to settle allegations that it had violated rules about its recruiting practices. The school did not admit any wrongdoing.
"I think maybe the whole orchard is contaminated," Harkin said. "There's a systemic problem with the system itself that needs to be addressed."University of Phoenix Recruiter's False Promise
ABC News wanted to know firsthand whether what Dalmier said happened to her, would happen to us, so we sent one of our producers undercover to meet with a University of Phoenix recruiter.
Our producer told the recruiter, who was working out of an office in Houston, Texas, that he aspired to be a teacher and planned to live in either Texas or New York. The recruiter told him to enroll in the Bachelor's of Science in Education program, and with that degree and some student teaching, he would be set.
Producer: I just want to understand clearly. I can go to University of Phoenix, do my bachelor's degree, and 100 percent for sure I can go back to either Texas, or New York and I can sit for those exams and once I finish those exams...I can teach.
Recruiter: Then you can become a teacher. Yes. That is true. What's your e-mail address?
Despite her assurances, the recruiter's claim was not true. Even with successful completion of the required certification testing, a degree from the University of Phoenix does not guarantee a teaching certificate in either of those states.
When we confronted Dr. William Pepicello, president of the University of Phoenix, about the recruiter's false promise, he said it was "indefensible."
"It's wrong. Can we do better? Absolutely. Do we train our people to give that kind of misadvice? Absolutely not. And we can do better, we will do better, you know, we already have some initiatives that we talked about that we're putting in place because at the end of the day, we have to get it right."
But this was not the first time that the university's recruiting practices have come under scrutiny. In December 2009, after two former employees came forward and accused the university of violating federal financial aid regulations with its recruiting practices, without admitting wrongdoing the school agreed to pay $67.5 million to resolve the accusations. The two whistleblowers received $19 million in the settlement.
When asked if the 2009 settlement was a sign that "we got caught," Pepicello disagreed.
"No, I wouldn't say it's proof that we got caught. I mean, it's certainly proof that we weren't doing as well as we could. We could do better," he said.Recruiter Tells Student to Borrow to the Max
The recruiter also told our undercover producer he could take out as much as $35,000 in federal financial aid to pay for school. She also said that there might even be some money left over after tuition was paid.
Recruiter: I tell students to take out the max and whatever you don't need or you don't use then use it [for whatever]. But it's easier to take out more than you need and send back the excess versus you didn't take out enough.
Producer: What are the kinds of things though? I mean in terms of like that I could use it for? I mean, what if I just...because you're going to have to have money to walk around.
Recruiter: They don't care. Right. They don't. They just tell you use it for educational purposes.
Producer: And they don't ...They don't what?
Recruiter: No one follows up. No one says, What happened to this money? You received a check for $562, where did you spend it?
Producer: It's your business.
The university president said that there was no excuse for a recruiter to push someone to borrow to the max.
'It's absolutely indefensible. It is not the way that I intend to run this university," Pepicello said.For-Profit Universities Contributing to Financial Crisis?
Experts say recruiters who are misleading students may only be the tip of the iceberg. Students who have attended for-profit schools are defaulting on their loans at an alarming rate, which experts say may be contributing to the next big financial crisis.
At the University of Phoenix's headquarters, the loan repayment rate was 44 percent, according to data from 2009 provided by the Department of Education; students at their Nellis Air Force location had a repayment rate of 36 percent. At the headquarters of Brown Mackie College, another for-profit school, the repayment rate was 27 percent.
Harris Miller, who heads the for-profit industry's lobby group, told Chris Cuomo that default rates at for profit schools are comparable to other schools which service similar student populations.
Recruiters from for-profit schools obtained $24 billion in student loan and grant money for the 2008-2009 school year, according to Government Accountability Office and Senate reports.
"These schools are marketing machines masquerading as universities," said Steve Eisman, a renowned hedge fund investor who made billions betting against the housing market, at a recent conference and during his testimony before the Senate on the for-profit sector. "I thought there would never again be an opportunity to be involved in the short side as an industry as social destructive and morally bankrupt as the sub-prime mortgage industry...Unfortunately, I was wrong."
Though for-profits get the lion's share of their tuition from financial aid, the default rates on loans for students who attended for profit schools are alarming. About 50 percent of the students at for-profits drop out, according to Eisman, so schools need to keep adding new students, and have to try to recruit just about anyone -- even those most vulnerable in society, he says.
Eisman's comments sparked controversy, and Miller lashed out at Eisman, calling his comparison of the career college sector to the subprime mortgage industry "silly" and "simplistic."
Miller also said Eisman's remarks must be considered in light of his alleged intentions as a short seller.
"It is no secret that the career education sector is under attack by short sellers, trial lawyers, self-styled consumer advocates, and some traditional academics. Although they should know better, these critics use anecdotes to generalize and to make sweeping condemnations of our sector," Miller said. "They seize on admittedly flawed government data to make the most extreme statistical arguments. They exploit the same small cadre of so-called third party experts to generate critical comments. And they recycle old news to give currency to new allegations. In short, they twist the truth to serve their self-interest."
But Eisman is not the only one leveling criticism at the for-profit education industry.University of Phoenix Recruiter Goes to Homeless Shelter
Benson Rawlins was considered homeless last year when he met two recruiters from the University of Phoenix, who gave three seminars at Y-Haven, a shelter for transitional men in Cleveland, Ohio, or in effect, a homeless shelter.
Rawlins doesn't have a GED, but said the recruiters had no qualms trying to sell him an expensive associate's degree.
"It seems like it is just too much all about money," he said, "Instead of helping someone get an education."
The university told ABC News it does not tolerate recruitment at facilities like Y-Haven.
"We can assure you that anyone who participated in the recruitment of residents from homeless facilities in Cleveland no longer works for the University," said Alex Clark, a spokesperson for the University of Phoenix. "Any such activity is strictly forbidden by our Code of Business Conduct and Ethics, and employees who violate this policy face disciplinary action up to and including termination."
Harris Miller said even though the schools serve an important role by providing higher education to students who wouldn't ordinarily get one, many schools' recruiting practices need to be changed.
Miller claimed that universities began to change even before the GAO's report on their misleading practices, including changing how recruiters are compensated (so they do not receive bonuses or prizes for recruiting students), offering "test drive" programs to help people figure out if higher education is for them and focusing more on consumer protection.
When asked why for-profit universities don't return money back to those who have been misled by their solicitations, Miller said: "There are other countries in the world like Canada which have a different system and it's something we're going to look at."
But Miller admitted that the industry has no plan in place to pay back those who are carrying a debt from for-profit schools.
Whatever the industry's plans for future, Dalmier said it won't help heal what happened.
"If they tell you something, investigate it before you enroll in their program. You really need to find out the truth and how to further your passion or your dream," she said. "That way, you don't end up like me."
After ABC News' interview with Pepicello, the University of Phoenix offered Dalmier a scholarship for a bachelor's degree of her choosing. Dalmier said she is considering their proposal.
Pepicello also said he plans to change the school's recruiting practices, especially the current model of compensation, and will be offering students a "test drive."
Click HERE to read a letter to ABC News from William Pepicello.
LOS ANGELES, April 10, 2012 /PRNewswire/ -- Distance learning, aka "correspondence courses," have been around for a couple of hundred years. You could learn to garden or sew by taking a course by mail, or study a foreign language. Today, most college educations provide some degree of online study, even when the student attends a brick-and-mortar institution full time. What makes Advanced College in South Gate, CA truly cutting edge is that an Associate's degree is available to you all-online and fully transferable to an accredited University where you can get a Bachelor's. But for this online course you never even have to set foot in the United States to earn your degree from a career college in Los Angeles.
The Associate's programs available at Advanced College are in highly lucrative and life-enriching fields. Take online courses and get a degree in accounting, health management, business administration, or medical assistance. These programs can be taken from out of state, or, indeed, a foreign country. Traveling as a student can be quite costly, and taking these courses online can mean massive savings.
Students of vocational schools in Los Angeles who are in need of financial aid must be located in the U.S., but at Advanced College, the tuition is already quite low. Financial aid, for those who qualify, is often less of a concern for Advanced College students, who have reported saving over $10,000 on tuition alone. Additionally, Kaplan University, an accredited institution offering numerous Bachelor's degree programs, fully accepts credits from students who transfer from Advanced College. What's more, Kaplan offers 10% off your tuition for transferring from Advanced College. This means a choice to study at Advanced College is a choice for a more financially secure future.
If your plans for an education involve immersion in American culture, and you would prefer to travel for your degree, Kaplan's I20 visa assistance means you'll avoid many of the challenges students face when they study abroad. Advanced College is fully certified by the Department of Homeland Security to perform this service, which places them among a select few trade schools in Los Angeles that can offer help with student visas. And if your plans change, and you want to move to the U.S. after you've already started with an online program, Advanced College will still perform this I20 service up till your last semester as a student. This is great news if you decide you want to transfer to Kaplan University.
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PR Submitted by www.cyberset.com
SOURCE Advanced College
Girard, J. P., Moussion, C. & Forster, R. HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes. Nat. Rev. Immunol. 12, 762–773 (2012).
Randolph, G. J. & Miller, N. E. Lymphatic transport of high-density lipoproteins and chylomicrons. J. Clin. Invest. 124, 929–935 (2014).
Miller, N. E. et al. Secretion of adipokines by human adipose tissue in vivo: partitioning between capillary and lymphatic transport. Am. J. Physiol. Endocrinol. Metab. 301, E659–E667 (2011).
Wiig, H. & Swartz, M. A. Interstitial fluid and lymph formation and transport: physiological regulation and roles in inflammation and cancer. Physiol. Rev. 92, 1005–1060 (2012).
Starling, E. H. On the absorption of fluids from the connective tissue spaces. J. Physiol. 19, 312–326 (1896).
Levick, J. R. & Michel, C. C. Microvascular fluid exchange and the revised Starling principle. Cardiovasc. Res. 87, 198–210 (2010).
Mortimer, P. S. & Rockson, S. G. New developments in clinical aspects of lymphatic disease. J. Clin. Invest. 124, 915–921 (2014).
Card, C. M., Yu, S. S. & Swartz, M. A. Emerging roles of lymphatic endothelium in regulating adaptive immunity. J. Clin. Invest. 124, 943–952 (2014).
Pabst, O. & Mowat, A. M. Oral tolerance to food protein. Mucosal Immunol. 5, 232–239 (2012).
Lichtenstein, L. et al. Angptl4 protects against severe proinflammatory effects of saturated fat by inhibiting fatty acid uptake into mesenteric lymph node macrophages. Cell. Metabolism 12, 580–592 (2010).
Macpherson, A. J. & Smith, K. Mesenteric lymph nodes at the center of immune anatomy. J. Exp. Med. 203, 497–500 (2006).
Dixon, J. B. Lymphatic lipid transport: sewer or subway? Trends Endocrinol. Metab. 21, 480–487 (2010).
Martel, C. et al. Lymphatic vasculature mediates macrophage reverse cholesterol transport in mice. J. Clin. Invest. 123, 1571–1579 (2013). A report on the important role of lymphatic vessels in facilitating HDL-mediated reverse cholesterol transport from tissues and atherosclerotic plaques to the systemic circulation, ultimately for excretion via the liver.
Lim, H. Y. et al. Lymphatic vessels are essential for the removal of cholesterol from peripheral tissues by SR-BI-mediated transport of HDL. Cell. Metabolism 17, 671–684 (2013). This article confirms the important role of lymphatic vessels in facilitating HDL-mediated reverse cholesterol transport from tissues, and provides evidence that HDL enters the lymphatics by active transcytosis across LECs via SRB1.
Harvey, N. L. The link between lymphatic function and adipose biology. Ann. NY Acad. Sci. 1131, 82–88 (2008).
Pond, C. M. Adipose tissue and the immune system. Prostaglandins Leukot. Essent. Fatty Acids 73, 17–30 (2005).
Harvey, N. L. et al. Lymphatic vascular defects promoted by Prox1 haploinsufficiency cause adult-onset obesity. Nat. Genet. 37, 1072–1081 (2005). This report highlights the links between lymphatics and adipose function and the development of obesity.
Sawane, M. et al. Apelin inhibits diet-induced obesity by enhancing lymphatic and blood vessel integrity. Diabetes 62, 1970–1980 (2013).
Blum, K. S. et al. Chronic high-fat diet impairs collecting lymphatic vessel function in mice. PLoS ONE 9, e94713 (2014).
Arngrim, N., Simonsen, L., Holst, J. J. & Bulow, J. Reduced adipose tissue lymphatic drainage of macromolecules in obese subjects: a possible link between obesity and local tissue inflammation? Int. J. Obes. 37, 748–750 (2013).
Savetsky, I. L. et al. Obesity increases inflammation and impairs lymphatic function in a mouse model of lymphedema. Am. J. Physiol. Heart Circ. Physiol. 307, H165–H172 (2014).
Weitman, E. S. et al. Obesity impairs lymphatic fluid transport and dendritic cell migration to lymph nodes. PLoS ONE 8, e70703 (2013).
Kim, C. S. et al. Visceral fat accumulation induced by a high-fat diet causes the atrophy of mesenteric lymph nodes in obese mice. Obesity 16, 1261–1269 (2008).
Alitalo, K. The lymphatic vasculature in disease. Nat. Med. 17, 1371–1380 (2011). A review of advances in our current understanding of the role of lymphatics in pathological change and disease.
Kesler, C. T., Liao, S., Munn, L. L. & Padera, T. P. Lymphatic vessels in health and disease. Wiley Interdiscip. Rev. Syst. Biol. Med. 5, 111–124 (2013).
Wang, Y. & Oliver, G. Current views on the function of the lymphatic vasculature in health and disease. Genes Dev. 24, 2115–2126 (2010).
Swartz, M. A. & Lund, A. W. Lymphatic and interstitial flow in the tumour microenvironment: linking mechanobiology with immunity. Nat. Rev. Cancer 12, 210–219 (2012).
Dieterich, L. C., Seidel, C. D. & Detmar, M. Lymphatic vessels: new targets for the treatment of inflammatory diseases. Angiogenesis 17, 359–371 (2014).
Proulx, S. T. et al. Expansion of the lymphatic vasculature in cancer and inflammation: new opportunities for in vivo imaging and drug delivery. J. Control. Release 172, 550–557 (2013).
von der Weid, P. Y., Rehal, S. & Ferraz, J. G. Role of the lymphatic system in the pathogenesis of Crohn's disease. Curr. Opin. Gastroenterol. 27, 335–341 (2011).
Alessio, S. et al. VEGF-C-dependent stimulation of lymphatic function ameliorates experimental inflammatory bowel disease. J. Clin. Invest. 124, 3863–3878 (2014).
Huggenberger, R. et al. An important role of lymphatic vessel activation in limiting acute inflammation. Blood 117, 4667–4678 (2011).
Zhang, Q. et al. Increased lymphangiogenesis in joints of mice with inflammatory arthritis. Arthritis Res. Ther. 9, R118 (2007).
Baluk, P. et al. TNF-α drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. J. Clin. Invest. 119, 2954–2964 (2009).
Machnik, A. et al. Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism. Nat. Med. 15, 545–552 (2009).
Ribera, J. et al. Increased nitric oxide production in lymphatic endothelial cells causes impairment of lymphatic drainage in cirrhotic rats. Gut 62, 138–145 (2012).
Jones, D. & Min, W. An overview of lymphatic vessels and their emerging role in cardiovascular disease. J. Cardiovasc. Dis. Res. 2, 141–152 (2011).
Fletcher, C. V. et al. Persistent HIV-1 replication is associated with lower antiretroviral drug concentrations in lymphatic tissues. Proc. Natl Acad. Sci. USA 111, 2307–2312 (2014). The data presented in this article links persistent HIV replication with low antiretroviral drug concentrations in lymphatic tissues in humans. Increasing drug distribution to lymph may therefore provide a treatment benefit.
Pantaleo, G. et al. Lymphoid organs function as major reservoirs for human-immunodeficiency-virus. Proc. Natl Acad. Sci. USA 88, 9838–9842 (1991).
Giannini, C. et al. Association between persistent lymphatic infection by hepatitis C virus after antiviral treatment and mixed cryoglobulinemia. Blood 111, 2943–2945 (2008).
Bennuru, S. & Nutman, T. B. Lymphangiogenesis and lymphatic remodeling induced by filarial parasites: implications for pathogenesis. PLoS Pathog. 5, e1000688 (2009).
Feldmann, H. & Geisbert, T. W. Ebola haemorrhagic fever. Lancet 377, 849–862 (2011).
Deitch, E. A. Gut lymph and lymphatics: a source of factors leading to organ injury and dysfunction. Ann. NY Acad. Sci. 1207, E103–E111 (2010).
Kerjaschki, D. et al. Lymphatic endothelial progenitor cells contribute to de novo lymphangiogenesis in human renal transplants. Nat. Med. 12, 230–234 (2006).
Wang, X. et al. Mechanism of oral tolerance induction to therapeutic proteins. Adv. Drug Deliv. Rev. 65, 759–773 (2013).
Swartz, M. A., Hirosue, S. & Hubbell, J. A. Engineering approaches to immunotherapy. Sci. Transl. Med. 4, 148rv9 (2012).
Trevaskis, N. L., Charman, W. N. & Porter, C. J. Lipid-based delivery systems and intestinal lymphatic drug transport: a mechanistic update. Adv. Drug Deliv. Rev. 60, 702–716 (2008).
Ryan, G. M., Kaminskas, L. M. & Porter, C. J. Nano-chemotherapeutics: maximising lymphatic drug exposure to improve the treatment of lymph-metastatic cancers. J. Control. Release 193, 241–256 (2014).
Yáñez, J. A., Wang, S. W. J., Knemeyer, I. W., Wirth, M. A. & Alton, K. B. Intestinal lymphatic transport for drug delivery. Adv. Drug Deliv. Rev. 63, 923–942 (2011).
Supersaxo, A., Hein, W. R. & Steffen, H. Effect of molecular-weight on the lymphatic absorption of water-soluble compounds following subcutaneous administration. Pharm. Res. 7, 167–169 (1990). The first paper to describe the relationship between molecular mass of proteins and lymphatic uptake from interstitial injection sites in sheep.
Irvine, D. J., Swartz, M. A. & Szeto, G. L. Engineering synthetic vaccines using cues from natural immunity. Nat. Mater. 12, 978–990 (2013).
Charman, S. A., McLennan, D. N., Edwards, G. A. & Porter, C. J. H. Lymphatic absorption is a significant contributor to the subcutaneous bioavailability of insulin in a sheep model. Pharm. Res. 18, 1620–1626 (2001).
Charman, S. A., Segrave, A. M., Edwards, G. A. & Porter, C. J. H. Systemic availability and lymphatic transport of human growth hormone administered by subcutaneous injection. J. Pharm. Sci. 89, 168–177 (2000).
Kota, J. et al. Lymphatic absorption of subcutaneously administered proteins: influence of different injection sites on the absorption of darbepoetin alfa using a sheep model. Drug Metab. Dispos. 35, 2211–2217 (2007).
McLennan, D. et al. Pharmacokinetic model to describe the lymphatic absorption of r-methu-leptin after subcutaneous injection to sheep. Pharm. Res. 20, 1156–1162 (2003).
McLennan, D. et al. The absorption of darbepoetin alfa occurs predominantly via the lymphatics following subcutaneous administration to sheep. Pharm. Res. 23, 2060–2066 (2006).
McLennan, D. N. et al. Lymphatic absorption is the primary contributor to the systemic availability of epoetin alfa following subcutaneous administration to sheep. J. Pharmacol. Exp. Ther. 313, 345–351 (2005).
Oussoren, C., Zuidema, J., Crommelin, D. J. & Storm, G. Lymphatic uptake and biodistribution of liposomes after subcutaneous injection. II. Influence of liposomal size, lipid composition and lipid dose. Biochim. Biophys. Acta 1328, 261–272 (1997). The first paper to establish the influence of size and composition on lymphatic uptake and retention of model delivery systems (liposomes).
Reddy, S. T. et al. Exploiting lymphatic transport and complement activation in nanoparticle vaccines. Nat. Biotech. 25, 1159–1164 (2007).
Reed, A. L., Rowson, S. A. & Dixon, J. B. Demonstration of ATP-dependent, transcellular transport of lipid across the lymphatic endothelium using an in vitro model of the lacteal. Pharm. Res. 30, 3271–3280 (2013).
Laakkonen, P. et al. Antitumor activity of a homing peptide that targets tumor lymphatics and tumor cells. Proc. Natl Acad. Sci. USA 101, 9381–9386 (2004).
Laakkonen, P., Porkka, K., Hoffman, J. A. & Ruoslahti, E. A tumor-homing peptide with a targeting specificity related to lymphatic vessels. Nat. Med. 8, 751–755 (2002).
Parker, J. C., Gilchrist, S. & Cartledge, J. T. Plasma–lymph exchange and interstitial distribution volumes of charged macromolecules in the lung. J. Appl. Physiol. 59, 1128–1136 (1985).
Stylianopoulos, T. et al. Diffusion of particles in the extracellular matrix: the effect of repulsive electrostatic interactions. Biophys. J. 99, 1342–1349 (2010).
Kaminskas, L. M. et al. PEGylation of polylysine dendrimers improves absorption and lymphatic targeting following SC administration in rats. J. Control. Release 140, 108–116 (2009). This article shows that PEGylation of the therapeutic protein interferon-α2 increases lymphatic distribution and ultimately increases therapeutic efficacy against a lymph-resident cancer.
Rao, D. A., Forrest, M. L., Alani, A. W., Kwon, G. S. & Robinson, J. R. Biodegradable PLGA based nanoparticles for sustained regional lymphatic drug delivery. J. Pharm. Sci. 99, 2018–2031 (2010).
Harvey, A. J. et al. Microneedle-based intradermal delivery enables rapid lymphatic uptake and distribution of protein drugs. Pharm. Res. 28, 107–116 (2011).
Lambert, P. H. & Laurent, P. E. Intradermal vaccine delivery: will new delivery systems transform vaccine administration? Vaccine 26, 3197–3208 (2008).
Nicolas, J.-F. & Guy, B. Intradermal, epidermal and transcutaneous vaccination: from immunology to clinical practice. Expert Rev. Vaccines 7, 1201–1214 (2008).
Bocci, V., Pessina, G. P., Paulesu, L. & Nicoletti, C. The lymphatic route. VI. Distribution of recombinant interferon-α2 in rabbit and pig plasma and lymph. J. Biolog. Response Mod. 7, 390–400 (1988).
Feng, L. et al. Roles of dextrans on improving lymphatic drainage for liposomal drug delivery system. J. Drug Target. 18, 168–178 (2010).
Pessina, G. P., Bocci, V., Carraro, F., Naldini, A. & Paulesu, L. The lymphatic route. IX. Distribution of recombinant interferon-α 2 administered subcutaneously with oedematogenic drugs. Physiol. Res. 42, 243–250 (1993).
Liu, H. et al. Structure-based programming of lymph-node targeting in molecular vaccines. Nature 507, 519–522 (2014). A pioneering article that uses targeted delivery to the lymphatics to enhance vaccination. This was achieved by the conjugation of peptides to lipids that bind to albumin and 'hitchhike' onto transport pathways from the interstitium into the lymphatics.
Jiang, G. et al. Hyaluronic acid–polyethyleneimine conjugate for target specific intracellular delivery of siRNA. Biopolymers 89, 635–642 (2008).
Fogal, V., Zhang, L., Krajewski, S. & Ruoslahti, E. Mitochondrial/cell-surface protein p32/gC1qR as a molecular target in tumor cells and tumor stroma. Cancer Res. 68, 7210–7218 (2008).
Karmali, P. P. et al. Targeting of albumin-embedded paclitaxel nanoparticles to tumors. Nanomedicine 5, 73–82 (2009).
Luo, G. et al. LyP-1-conjugated nanoparticles for targeting drug delivery to lymphatic metastatic tumors. Int. J. Pharm. 385, 150–156 (2010).
Yan, Z. et al. LyP-1-conjugated PEGylated liposomes: a carrier system for targeted therapy of lymphatic metastatic tumor. J. Control. Release 157, 118–125 (2012).
Desgrosellier, J. S. & Cheresh, D. A. Integrins in cancer: biological implications and therapeutic opportunities. Nat. Rev. Cancer 10, 9–22 (2010).
Andorko, J., Hess, K. & Jewell, C. Harnessing biomaterials to engineer the lymph node microenvironment for immunity or tolerance. AAPS J. 17, 323–338 (2014). A summary of the mechanisms by which materials can be engineered to promote delivery to cells within the lymphatics to enhance vaccination and tolerance induction.
Zeng, Q. et al. Cationic micelle delivery of Trp2 peptide for efficient lymphatic draining and enhanced cytotoxic T-lymphocyte responses. J. Control. Release 200, 1–12 (2015).
Wang, C. et al. Lymphatic-targeted cationic liposomes: a robust vaccine adjuvant for promoting long-term immunological memory. Vaccine 32, 5475–5483 (2014).
Azad, A. K., Rajaram, M. V. & Schlesinger, L. S. Exploitation of the macrophage mannose receptor (CD206) in infectious disease diagnostics and therapeutics. J. Cytol. Mol. Biol. 1, 1000003 (2014).
Kwon, Y. J., James, E., Shastri, N. & Fréchet, J. M. J. In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles. Proc. Natl Acad. Sci. USA 102, 18264–18268 (2005).
Dahlberg, A. M. et al. The lymphatic system plays a major role in the intravenous and subcutaneous pharmacokinetics of trastuzumab in rats. Mol. Pharm. 11, 496–504 (2014).
Ryan, G. M. et al. PEGylated polylysine dendrimers increase lymphatic exposure to doxorubicin when compared to PEGylated liposomal and solution formulations of doxorubicin. J. Control. Release 172, 128–136 (2013).
Tseng, Y. C., Xu, Z., Guley, K., Yuan, H. & Huang, L. Lipid–calcium phosphate nanoparticles for delivery to the lymphatic system and SPECT/CT imaging of lymph node metastases. Biomaterials 35, 4688–4698 (2014).
Iliff, J. J. et al. Brain-wide pathway for waste clearance captured by contrast-enhanced MRI. J. Clin. Invest. 123, 1299–1309 (2013).
Iliff, J. J. et al. A paravascular pathway facilitates CSF flow through the brain parenchyma and the clearance of interstitial solutes, including amyloid β. Sci. Transl. Med. 4, 147ra111 (2012). This article provides the first description of the glymphatic system — a brain-wide paravascular pathway for CSF and ISF exchange that facilitates the clearance of solutes and waste from the brain.
Iliff, J. J. et al. Cerebral arterial pulsation drives paravascular CSF-interstitial fluid exchange in the murine brain. J. Neurosci. 33, 18190–18199 (2013).
Xie, L. et al. Sleep drives metabolite clearance from the adult brain. Science 342, 373–377 (2013).
Yang, L. et al. Evaluating glymphatic pathway function utilizing clinically relevant intrathecal infusion of CSF tracer. J. Transl. Med. 11, 107 (2013).
Aspelund, A., et al. A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules. J. Exp. Med. 212, 991–999 (2015).
Louveau, A., et al. Structural and functional features of central nervous system lymphatic vessels. Nature 523, 337–341 (2015).
Shackleford, D., Porter, C. H. & Charman, W. in Prodrugs Vol. 5 (eds Stella, V. et al.) 653–682 (Springer, 2007).
Lambert, D. M. Rationale and applications of lipids as prodrug carriers. Eur. J. Pharm. Sci. 11 (Suppl. 2), S15–S27 (2000).
Kunisawa, J., Kurashima, Y. & Kiyono, H. Gut-associated lymphoid tissues for the development of oral vaccines. Adv. Drug Deliv. Rev. 64, 523–530 (2012).
Bakhru, S. H., Furtado, S., Morello, A. P. & Mathiowitz, E. Oral delivery of proteins by biodegradable nanoparticles. Adv. Drug Deliv. Rev. 65, 811–821 (2013).
Florence, A. T. Nanoparticle uptake by the oral route: fulfilling its potential? Drug Discov. Today Technol. 2, 75–81 (2005).
Khoo, S. M., Shackleford, D. M., Porter, C. J., Edwards, G. A. & Charman, W. N. Intestinal lymphatic transport of halofantrine occurs after oral administration of a unit-dose lipid-based formulation to fasted dogs. Pharm. Res. 20, 1460–1465 (2003). This article uses a dog model to demonstrate the potential for even a single capsule of lipid to promote significant intestinal lymphatic drug transport.
Caliph, S. M., Charman, W. N. & Porter, C. J. Effect of short-, medium-, and long-chain fatty acid-based vehicles on the absolute oral bioavailability and intestinal lymphatic transport of halofantrine and assessment of mass balance in lymph-cannulated and non-cannulated rats. J. Pharm. Sci. 89, 1073–1084 (2000).
Trevaskis, N. L. et al. A mouse model to evaluate the impact of species, sex, and lipid load on lymphatic drug transport. Pharm. Res. 30, 3254–3270 (2013). An article describing a mesenteric lymph duct cannulated mouse model to evaluate intestinal lymphatic drug transport and provides a cross comparison of preclinical species.
Charman, W. N. & Stella, V. J. Estimating the maximum potential for intestinal lymphatic transport of lipophilic drug molecules. Int. J. Pharm. 34, 175–178 (1986). The first paper to suggest the importance of logP and lipid solubility in indicating the potential for drug absorption via the intestinal lymphatics.
Myers, R. A. & Stella, V. J. Factors affecting the lymphatic transport of penclomedine (NSC-338720), a lipophilic cytotoxic drug — comparison to DDT and hexachlorobenzene. Int. J. Pharm. 80, 51–62 (1992).
Trevaskis, N. L., Shanker, R. M., Charman, W. N. & Porter, C. J. The mechanism of lymphatic access of two cholesteryl ester transfer protein inhibitors (CP524,515 and CP532,623) and evaluation of their impact on lymph lipoprotein profiles. Pharm. Res. 27, 1949–1964 (2010).
Choo, E. F. et al. The role of lymphatic transport on the systemic bioavailability of the Bcl-2 protein family inhibitors navitoclax (ABT-263) and ABT-199. Drug Metab. Dispos. 42, 207–212 (2014). This article demonstrates significant intestinal lymphatic transport of a clinical drug candidate in dogs.
Gershkovich, P. et al. The role of molecular physicochemical properties and apolipoproteins in association of drugs with triglyceride-rich lipoproteins: in-silico prediction of uptake by chylomicrons. J. Pharm. Pharmacol. 61, 31–39 (2009).
Gershkovich, P. & Hoffman, A. Uptake of lipophilic drugs by plasma derived isolated chylomicrons: linear correlation with intestinal lymphatic bioavailability. Eur. J. Pharm. Sci. 26, 394–404 (2005).
Lawless, E., Griffin, B., O'Mahony, A. & O'Driscoll, C. Exploring the impact of drug properties on the extent of intestinal lymphatic transport — in vitro and in vivo studies. Pharm. Res. 32, 1817–1829 (2014).
Lu, Y. et al. Biomimetic reassembled chylomicrons as novel association model for the prediction of lymphatic transportation of highly lipophilic drugs via the oral route. Int. J. Pharm. 483, 69–76 (2015).
Holm, R. & Hoest, J. Successful in silico predicting of intestinal lymphatic transfer. Int. J. Pharm. 272, 189–193 (2004).
Lipinski, C. A., Lombardo, F., Dominy, B. W. & Feeney, P. J. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Adv. Drug Deliv. Rev. 46, 3–26 (2001).
Hopkins, A. L., Keseru, G. M., Leeson, P. D., Rees, D. C. & Reynolds, C. H. The role of ligand efficiency metrics in drug discovery. Nat. Rev. Drug Discov. 13, 105–121 (2014).
Han, S. et al. Targeted delivery of a model immunomodulator to the lymphatic system: comparison of alkyl ester versus triglyceride mimetic lipid prodrug strategies. J. Control. Release 177, 1–10 (2014). This article reports glyceride mimetic prodrugs that are more efficiently transported into the intestinal lymph following oral delivery compared with alkyl ester or amide prodrugs, and that they enhance drug delivery to MLNs.
Sugihara, J., Furuuchi, S., Nakano, K. & Harigaya, S. Studies on intestinal lymphatic absorption of drugs. I. Lymphatic absorption of alkyl ester derivatives and alpha-monoglyceride derivatives of drugs. J. Pharmacobiodyn. 11, 369–376 (1988).
Sugihara, J., Furuuchi, S., Ando, H., Takashima, K. & Harigaya, S. Studies on intestinal lymphatic absorption of drugs. II. Glyceride prodrugs for improving lymphatic absorption of naproxen and nicotinic-acid. J. Pharmacobiodyn. 11, 555–562 (1988).
Dahan, A. et al. The oral absorption of phospholipid prodrugs: in vivo and in vitro mechanistic investigation of trafficking of a lecithin–valproic acid conjugate following oral administration. J. Control. Release 126, 1–9 (2008).
Sakai, A., Mori, N., Shuto, S. & Suzuki, T. Deacylation-reacylation cycle: a possible absorption mechanism for the novel lymphotropic antitumor agent dipalmitoylphosphatidylfluorouridine in rats. J. Pharm. Sci. 82, 575–578 (1993).
Hussain, N., Jaitley, V. & Florence, A. T. Recent advances in the understanding of uptake of microparticulates across the gastrointestinal lymphatics. Adv. Drug Deliv. Rev. 50, 107–142 (2001).
Yun, Y., Cho, Y. W. & Park, K. Nanoparticles for oral delivery: targeted nanoparticles with peptidic ligands for oral protein delivery. Adv. Drug Deliv. Rev. 65, 822–832 (2013).
Pasetti, M. F., Simon, J. K., Sztein, M. B. & Levine, M. M. Immunology of gut mucosal vaccines. Immunol. Rev. 239, 125–148 (2011).
Attili-Qadri, S. et al. Oral delivery system prolongs blood circulation of docetaxel nanocapsules via lymphatic absorption. Proc. Natl Acad. Sci. USA 110, 17498–17503 (2013).
Pridgen, E. M. et al. Transepithelial transport of Fc-targeted nanoparticles by the neonatal Fc receptor for oral delivery. Sci. Transl. Med. 5, 213ra167 (2013).
Neutra, M. R. & Kozlowski, P. A. Mucosal vaccines: the promise and the challenge. Nat. Rev. Immunol. 6, 148–158 (2006).
Schulz, O. et al. Intestinal CD103+, but not CX3CR1+, antigen sampling cells migrate in lymph and serve classical dendritic cell functions. J. Exp. Med. 206, 3101–3114 (2009).
Rescigno, M. Intestinal dendritic cells. Adv. Immunol. 107, 109–138 (2010).
Niess, J. H. et al. CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance. Science 307, 254–258 (2005).
Clark, M. A., Hirst, B. H. & Jepson, M. A. Lectin-mediated mucosal delivery of drugs and microparticles. Adv. Drug Deliv. Rev. 43, 207–223 (2000).
Hussain, N. & Florence, A. Utilizing bacterial mechanisms of epithelial cell entry: invasin-induced oral uptake of latex nanoparticles. Pharm. Res. 15, 153–156 (1998).
Fievez, V. et al. Targeting nanoparticles to M cells with non-peptidic ligands for oral vaccination. Eur. J. Pharm. Biopharm. 73, 16–24 (2009).
Jin, Y. et al. Goblet cell-targeting nanoparticles for oral insulin delivery and the influence of mucus on insulin transport. Biomaterials 33, 1573–1582 (2012).
Reineke, J. J. et al. Unique insights into the intestinal absorption, transit, and subsequent biodistribution of polymer-derived microspheres. Proc. Natl Acad. Sci. USA 110, 13803–13808 (2013).
Desai, M. P., Labhasetwar, V., Amidon, G. L. & Levy, R. J. Gastrointestinal uptake of biodegradable microparticles: effect of particle size. Pharm. Res. 13, 1838–1845 (1996).
Jani, P., Halbert, G. W., Langridge, J. & Florence, A. T. Nanoparticle uptake by the rat gastrointestinal mucosa: quantitation and particle size dependency. J. Pharm. Pharmacol. 42, 821–826 (1990).
Ebel, J. A. Method for quantifying particle absorption from the small intestine of the mouse. Pharm. Res. 7, 848–851 (1990).
Jenkins, P. G. et al. The quantitation of the absorption of microparticles into the intestinal lymph of Wistar rats. Int. J. Pharm. 102, 261–266 (1994).
Lefevre, M. E., Joel, D. D. & Schidlovsky, G. Retention of ingested latex particles in Peyer's patches of germfree and conventional mice. Proc. Soc. Exp. Biol. Med. 179, 522–528 (1985).
Hussain, N., Jani, P. U. & Florence, A. T. Enhanced oral uptake of tomato lectin-conjugated nanoparticles in the rat. Pharm. Res. 14, 613–618 (1997).
Ralay-Ranaivo, B. et al. Novel self assembling nanoparticles for the oral administration of fondaparinux: synthesis, characterization and in vivo evaluation. J. Control. Release 194, 323–331 (2014).
Zhang, N. et al. Lectin-modified solid lipid nanoparticles as carriers for oral administration of insulin. Int. J. Pharm. 327, 153–159 (2006).
Florence, A. T., Sakthivel, T. & Toth, I. Oral uptake and translocation of a polylysine dendrimer with a lipid surface. J. Control. Release 65, 253–259 (2000).
Ryan, G. M. et al. Pulmonary administration of PEGylated polylysine dendrimers: absorption from the lung versus retention within the lung is highly size-dependent. Mol. Pharm. 10, 2986–2995 (2013).
Lycke, N. Recent progress in mucosal vaccine development: potential and limitations. Nat. Rev. Immunol. 12, 592–605 (2012).
Meeusen, E. N. Exploiting mucosal surfaces for the development of mucosal vaccines. Vaccine 29, 8506–8511 (2011).
Stano, A. et al. PPS nanoparticles as versatile delivery system to induce systemic and broad mucosal immunity after intranasal administration. Vaccine 29, 804–812 (2011).
Stano, A., Nembrini, C., Swartz, M. A., Hubbell, J. A. & Simeoni, E. Nanoparticle size influences the magnitude and quality of mucosal immune responses after intranasal immunization. Vaccine 30, 7541–7546 (2012).
Rytting, E., Nguyen, J., Wang, X. & Kissel, T. Biodegradable polymeric nanocarriers for pulmonary drug delivery. Expert Opin. Drug Deliv. 5, 629–639 (2008).
Patton, J. S., Fishburn, C. S. & Weers, J. G. The lungs as a portal of entry for systemic drug delivery. Proc. Am. Thorac. Soc. 1, 338–344 (2004).
Schraufnagel, D. E. Lung lymphatic anatomy and correlates. Pathophysiology 17, 337–343 (2010).
Pabst, R. & Tschernig, T. Bronchus-associated lymphoid tissue. Am. J. Respir. Cell. Mol. Biol. 43, 137–141 (2010).
Geiser, M. Update on macrophage clearance of inhaled micro- and nanoparticles. J. Aerosol Med. Pulm. Drug Deliv. 23, 207–217 (2010).
Wanner, A., Salathe, M. & O'Riordan, T. G. Mucociliary clearance in the airways. Am. J. Respir. Crit. Care Med. 154, 1868–1902 (1996).
Kambouchner, M. & Bernaudin, J. F. Intralobular pulmonary lymphatic distribution in normal human lung using D2-40 antipodoplanin immunostaining. J. Histochem. Cytochem. 57, 643–648 (2009).
Botelho, M. F. et al. Visualization of deep lung lymphatic network using radioliposomes. Rev. Port. Pneumol. 17, 124–130 (in Portuguese) (2011).
Hanatani, K. et al. Molecular weight-dependent lymphatic transfer of fluorescein isothiocyanate-labeled dextrans after intrapulmonary administration and effects of various absorption enhancers on the lymphatic transfer of drugs in rats. J. Drug Target 3, 263–271 (1995).
Choi, H. S. et al. Rapid translocation of nanoparticles from the lung airspaces to the body. Nat. Biotech. 28, 1300–1303 (2010).
Li, A. V. et al. Generation of effector memory T cell-based mucosal and systemic immunity with pulmonary nanoparticle vaccination. Sci. Transl. Med. 5, 204ra130 (2013).
Videira, M. A. et al. Lymphatic uptake of pulmonary delivered radiolabelled solid lipid nanoparticles. J. Drug Target 10, 607–613 (2002).
Latimer, P. et al. Aerosol delivery of liposomal formulated paclitaxel and vitamin E analog reduces murine mammary tumor burden and metastases. Exp. Biol. Med. (Maywood) 234, 1244–1252 (2009).
Mohammad, A. K., Amayreh, L. K., Mazzara, J. M. & Reineke, J. J. Rapid lymph accumulation of polystyrene nanoparticles following pulmonary administration. Pharm. Res. 30, 424–434 (2013).
Mackay, C. R., Marston, W. L. & Dudler, L. Naive and memory T cells show distinct pathways of lymphocyte recirculation. J. Exp. Med. 171, 801–817 (1990).
Braun, A. Afferent lymph-derived T cells and DCs use different chemokine receptor CCR7-dependent routes for entry into the lymph node and intranodal migration. Nat. Immunol. 12, 879–887 (2011).
Moghimi, S. M. & Bonnemain, B. Subcutaneous and intravenous delivery of diagnostic agents to the lymphatic system: applications in lymphoscintigraphy and indirect lymphography. Adv. Drug Deliv. Rev. 37, 295–312 (1999).
Sensken, S.-C., Bode, C. & Gräler, M. H. Accumulation of fingolimod (FTY720) in lymphoid tissues contributes to prolonged efficacy. J. Pharmacol. Exp. Ther. 328, 963–969 (2009).
Manolova, V. et al. Nanoparticles target distinct dendritic cell populations according to their size. Eur. J. Immunol. 38, 1404–1413 (2008). The first demonstration of the relative importance of direct drainage versus transport after cellular uptake compared to lymph node uptake of particles after interstitial injection.
Moghimi, S. M. et al. Surface engineered nanospheres with enhanced drainage into lymphatics and uptake by macrophages of the regional lymph nodes. FEBS Lett. 344, 25–30 (1994).
Oussoren, C. et al. Lymphatic uptake and biodistribution of liposomes after subcutaneous injection: IV. Fate of liposomes in regional lymph nodes. Biochim. Biophys. Acta 1370, 259–272 (1998).
Reddy, S. T., Rehor, A., Schmoekel, H. G., Hubbell, J. A. & Swartz, M. A. In vivo targeting of dendritic cells in lymph nodes with poly(propylene sulfide) nanoparticles. J. Control. Release 112, 26–34 (2006). An article demonstrating the size dependency of lymphatic uptake, lymph node retention and lymph node dendritic cell uptake of nanoparticles.
Sixt, M. et al. The conduit system transports soluble antigens from the afferent lymph to resident dendritic cells in the T cell area of the lymph node. Immunity 22, 19–29 (2005).
Caserta, S., Alessi, P., Guarnerio, J., Basso, V. & Mondino, A. Synthetic CD4+ T cell-targeted antigen-presenting cells elicit protective antitumor responses. Cancer Res. 68, 3010–3018 (2008).
Moon, J. J. et al. Enhancing humoral responses to a malaria antigen with nanoparticle vaccines that expand Tfh cells and promote germinal center induction. Proc. Natl Acad. Sci. USA 109, 1080–1085 (2012). A key paper demonstrating that targeting delivery to lymph nodes enhances vaccination. See also references 207 and 209.
Oussoren, C. & Storm, G. Liposomes to target the lymphatics by subcutaneous administration. Adv. Drug Deliv. Rev. 50, 143–156 (2001).
Takakura, Y., Matsumoto, S., Hashida, M. & Sezaki, H. Enhanced lymphatic delivery of mitomycin C conjugated with dextran. Cancer Res. 44, 2505–2510 (1984).
Kim, C. K. & Han, J. H. Lymphatic delivery and pharmacokinetics of methotrexate after intramuscular injection of differently charged liposome-entrapped methotrexate to rats. J. Microencapsul. 12, 437–446 (1995).
Kaminskas, L. M. et al. Methotrexate-conjugated PEGylated dendrimers show differential patterns of deposition and activity in tumor-burdened lymph nodes after intravenous and subcutaneous administration in rats. Mol. Pharm. 12, 432–443 (2015).
Nune, S. K., Gunda, P., Majeti, B. K., Thallapally, P. K. & Forrest, M. L. Advances in lymphatic imaging and drug delivery. Adv. Drug Deliv. Rev. 63, 876–885 (2011).
Shackleford, D. M. et al. Contribution of lymphatically transported testosterone undecanoate to the systemic exposure of testosterone after oral administration of two andriol formulations in conscious lymph duct-cannulated dogs. J. Pharmacol. Exp. Ther. 306, 925–933 (2003).
White, K. L. et al. Lymphatic transport of methylnortestosterone undecanoate (MU) and the bioavailability of methylnortestosterone are highly sensitive to the mass of coadministered lipid after oral administration of MU. J. Pharmacol. Exp. Ther. 331, 700–709 (2009).
Surampudi, P. et al. Single, escalating dose pharmacokinetics, safety and food effects of a new oral androgen dimethandrolone undecanoate in man: a prototype oral male hormonal contraceptive. Andrology 2, 579–587 (2014).
Khoo, S. M., Edwards, G. A., Porter, C. J. H. & Charman, W. N. A conscious dog model for assessing the absorption, enterocyte-based metabolism, and intestinal lymphatic transport of halofantrine. J. Pharm. Sci. 90, 1599–1607 (2001).
Trevaskis, N. L. et al. Intestinal lymphatic transport enhances the post-prandial oral bioavailability of a novel cannabinoid receptor agonist via avoidance of first-pass metabolism. Pharm. Res. 26, 1486–1495 (2009).
Trevaskis, N. L., Porter, C. J. & Charman, W. N. An examination of the interplay between enterocyte-based metabolism and lymphatic drug transport in the rat. Drug Metab. Dispos. 34, 729–733 (2006).
Zhang, Z. et al. A self-assembled nanocarrier loading teniposide improves the oral delivery and drug concentration in tumor. J. Control. Release 166, 30–37 (2013).
Garzonaburbeh, A., Poupaert, J. H., Claesen, M., Dumont, P. & Atassi, G. 1,3-dipalmitoylglycerol ester of chlorambucil as a lymphotropic, orally administrable anti-neoplastic agent. J. Med. Chem. 26, 1200–1203 (1983).
Kaminskas, L. M. et al. PEGylation of interferon α2 improves lymphatic exposure after subcutaneous and intravenous administration and improves antitumour efficacy against lymphatic breast cancer metastases. J. Control. Release 168, 200–208 (2013).
Li, S., Goins, B., Hrycushko, B. A., Phillips, W. T. & Bao, A. Feasibility of eradication of breast cancer cells remaining in postlumpectomy cavity and draining lymph nodes following intracavitary injection of radioactive immunoliposomes. Mol. Pharm. 9, 2513–2522 (2012).
Cai, S., Xie, Y., Davies, N. M., Cohen, M. S. & Forrest, M. L. Carrier-based intralymphatic cisplatin chemotherapy for the treatment of metastatic squamous cell carcinoma of the head and neck. Ther. Delivery 1, 237–245 (2010).
Qin, L. et al. Polymeric micelles for enhanced lymphatic drug delivery to treat metastatic tumors. J. Control. Release 171, 133–142 (2013).
Rafi, M. et al. Polymeric micelles incorporating (1,2-diaminocyclohexane)platinum (II) suppress the growth of orthotopic scirrhous gastric tumors and their lymph node metastasis. J. Control. Release 159, 189–196 (2012).
Kourtis, I. C. et al. Peripherally administered nanoparticles target monocytic myeloid cells, secondary lymphoid organs and tumors in mice. PLoS ONE 8, e61646 (2013).
Liu, R. et al. Prevention of nodal metastases in breast cancer following the lymphatic migration of paclitaxel-loaded expansile nanoparticles. Biomaterials 34, 1810–1819 (2013).
Cai, S., Xie, Y., Bagby, T. R., Cohen, M. S. & Forrest, M. L. Intralymphatic chemotherapy using a hyaluronan–cisplatin conjugate. J. Surg. Res. 147, 247–252 (2008).
Akamo, Y. et al. Chemotherapy targeting regional lymph nodes by gastric submucosal injection of liposomal adriamycin in patients with gastric carcinoma. Jpn J. Cancer Res. 85, 652–658 (1994).
Khullar, O. V. et al. Nanoparticle migration and delivery of paclitaxel to regional lymph nodes in a large animal model. J. Am. Coll. Surg. 214, 328–337 (2012).
Ling, R. et al. Lymphatic chemotherapy induces apoptosis in lymph node metastases in a rabbit breast carcinoma model. J. Drug Target. 13, 137–142 (2005).
Yang, F. et al. Magnetic functionalised carbon nanotubes as drug vehicles for cancer lymph node metastasis treatment. Eur. J. Cancer 47, 1873–1882 (2011).
Zhao, C. et al. Local targeted therapy of liver metastasis from colon cancer by galactosylated liposome encapsulated with doxorubicin. PLoS ONE 8, e73860 (2013).
Dane, K. Y. et al. Nano-sized drug-loaded micelles deliver payload to lymph node immune cells and prolong allograft survival. J. Control. Release 156, 154–160 (2011).
Trevaskis, N. L., Charman, W. N. & Porter, C. J. Targeted drug delivery to lymphocytes: a route to site-specific immunomodulation? Mol. Pharm. 7, 2297–2309 (2010).
Okanobo, A., Chauhan, S. K., Dastjerdi, M. H., Kodati, S. & Dana, R. Efficacy of topical blockade of interleukin-1 in experimental dry eye disease. Am. J. Ophthalmol. 154, 63–71 (2012).
Shinriki, S. et al. Interleukin-6 signalling regulates vascular endothelial growth factor-C synthesis and lymphangiogenesis in human oral squamous cell carcinoma. J. Pathol. 225, 142–150 (2011).
Polzer, K. et al. Tumour necrosis factor blockade increases lymphangiogenesis in murine and human arthritic joints. Ann. Rheum. Dis. 67, 1610–1616 (2008).
Pal, I. & Ramsey, J. D. The role of the lymphatic system in vaccine trafficking and immune response. Adv. Drug Deliv. Rev. 63, 909–922 (2011).
Woodruff, M. C. et al. Trans-nodal migration of resident dendritic cells into medullary interfollicular regions initiates immunity to influenza vaccine. J. Exp. Med. 211, 1611–1621 (2014).
Senti, G., Johansen, P. & Kundig, T. M. Intralymphatic immunotherapy. Curr. Opin. Allergy Clin. Immunol. 9, 537–543 (2009).
Senti, G. et al. Intralymphatic allergen administration renders specific immunotherapy faster and safer: a randomized controlled trial. Proc. Natl Acad. Sci. USA 105, 17908–17912 (2008). A clinical trial demonstrating the benefit of intralymphatic administration to induce allergen tolerance and reduce allergen-induced rhinoconjunctivitis.
Jewell, C. M., Bustamante López, S. C. & Irvine, D. J. In situ engineering of the lymph node microenvironment via intranodal injection of adjuvant-releasing polymer particles. Proc. Natl Acad. Sci. USA 108, 15745–15750 (2011).
Maloy, K. J. et al. Intralymphatic immunization enhances DNA vaccination. Proc. Natl Acad. Sci. USA 98, 3299–3303 (2001).
De Titta, A. et al. Nanoparticle conjugation of CpG enhances adjuvancy for cellular immunity and memory recall at low dose. Proc. Natl Acad. Sci. USA 110, 19902–19907 (2013).
Xu, Z. et al. Multifunctional nanoparticles co-delivering Trp2 peptide and CpG adjuvant induce potent cytotoxic T-lymphocyte response against melanoma and its lung metastasis. J. Control. Release 172, 259–265 (2013).
Moon, J. J. et al. Interbilayer-crosslinked multilamellar vesicles as synthetic vaccines for potent humoral and cellular immune responses. Nat. Mater. 10, 243–251 (2011).
St John, A. L., Chan, C. Y., Staats, H. F., Leong, K. W. & Abraham, S. N. Synthetic mast-cell granules as adjuvants to promote and polarize immunity in lymph nodes. Nat. Mater. 11, 250–257 (2012).
Jeanbart, L. et al. Enhancing efficacy of anticancer vaccines by targeted delivery to tumor-draining lymph nodes. Cancer Immunol. Res. 2, 436–447 (2014).
Thomas, S. N., Vokali, E., Lund, A. W., Hubbell, J. A. & Swartz, M. A. Targeting the tumor-draining lymph node with adjuvanted nanoparticles reshapes the anti-tumor immune response. Biomaterials 35, 814–824 (2014).
Kim, S. H., Lee, K. Y. & Jang, Y. S. Mucosal immune system and M cell-targeting strategies for oral mucosal vaccination. Immune Netw. 12, 165–175 (2012).
Zhu, Q. et al. Large intestine-targeted, nanoparticle-releasing oral vaccine to control genitorectal viral infection. Nat. Med. 18, 1291–1296 (2012).
Ballester, M. et al. Nanoparticle conjugation and pulmonary delivery enhance the protective efficacy of Ag85B and CpG against tuberculosis. Vaccine 29, 6959–6966 (2011).
Nembrini, C. et al. Nanoparticle conjugation of antigen enhances cytotoxic T-cell responses in pulmonary vaccination. Proc. Natl Acad. Sci. USA 108, E989–E997 (2011). This article demonstrates enhanced immunization and protection against influenza-ova infection via pulmonary administration of lymph node-targeted antigens in nanoparticles with CpG relative to administration of soluble antigens with CpG.
Faria, A. M. C. & Weiner, H. L. Oral tolerance: therapeutic implications for autoimmune diseases. Clin. Dev. Immunol. 13, 143–157 (2006).
Miller, S. D., Turley, D. M. & Podojil, J. R. Antigen-specific tolerance strategies for the prevention and treatment of autoimmune disease. Nat. Rev. Immunol. 7, 665–677 (2007).
Weiner, H. L., da Cunha, A. P., Quintana, F. & Wu, H. Oral tolerance. Immunol. Rev. 241, 241–259 (2011).
Scandling, J. D., Busque, S., Shizuru, J. A., Engleman, E. G. & Strober, S. Induced immune tolerance for kidney transplantation. N. Engl. J. Med. 365, 1359–1360 (2011).
Faria, A. M. C. & Weiner, H. L. Oral tolerance. Immunol. Rev. 206, 232–259 (2005).
Burks, A. W., Laubach, S. & Jones, S. M. Oral tolerance, food allergy, and immunotherapy: implications for future treatment. J. Allergy Clin. Immunol. 121, 1344–1350 (2008).
Worbs, T. et al. Oral tolerance originates in the intestinal immune system and relies on antigen carriage by dendritic cells. J. Exp. Med. 203, 519–527 (2006).
Spahn, T. W. et al. Mesenteric lymph nodes are critical for the induction of high-dose oral tolerance in the absence of Peyer's patches. Eur. J. Immunol. 32, 1109–1113 (2002).
Spahn, T. W. et al. Induction of oral tolerance to cellular immune responses in the absence of Peyer's patches. Eur. J. Immunol. 31, 1278–1287 (2001).
Kraus, T. A. et al. Induction of mucosal tolerance in Peyer's patch-deficient, ligated small bowel loops. J. Clin. Invest. 115, 2234–2243 (2005).
Fujihashi, K. et al. Peyer's patches are required for oral tolerance to proteins. Proc. Natl Acad. Sci. USA 98, 3310–3315 (2001).
Suzuki, H. et al. Ovalbumin-protein sigma 1 M-cell targeting facilitates oral tolerance with reduction of antigen-specific CD4+ T cells. Gastroenterology 135, 917–925 (2008).
Masuda, K., Horie, K., Suzuki, R., Yoshikawa, T. & Hirano, K. Oral delivery of antigens in liposomes with some lipid compositions modulates oral tolerance to the antigens. Microbiol. Immunol. 46, 55–58 (2002).
Kim, W. et al. Suppression of collagen-induced arthritis by single feeding of poilylactic-poilyglycolic acid entrapping immunodominant peptide of type II collagen: involvement of CD4+ IL-10+ T cells in Peyer's pathces. Ann. Rheum. Dis. 62, 168–168 (2003).
Goldmann, K., Hoffmann, J., Eckl, S., Spriewald, B. M. & Ensminger, S. M. Attenuation of transplant arteriosclerosis by oral feeding of major histocompatibility complex encoding chitosan-DNA nanoparticles. Transplant Immunol. 28, 9–13 (2013).
Pecquet, S. et al. Oral tolerance elicited in mice by β-lactoglobulin entrapped in biodegradable microspheres. Vaccine 18, 1196–1202 (2000).
Shirali, A. C. et al. Nanoparticle delivery of mycophenolic acid upregulates PD-L1 on dendritic cells to prolong murine allograft survival. Am. J. Transplant. 11, 2582–2592 (2011).
Maldonado, R. A. et al. Polymeric synthetic nanoparticles for the induction of antigen-specific immunological tolerance. Proc. Natl Acad. Sci. USA 112, E156–E165 (2015). Provides an innovative approach to enhance and prolong tolerance induction via the administration of 'tolerogenic' nanoparticles loaded with antigens and the tolerogenic immunomodulator rapamycin that are efficiently transport to lymphoid organs and captured by resident APCs.
Capini, C. et al. Antigen-specific suppression of inflammatory arthritis using liposomes. J. Immunol. 182, 3556–3565 (2009).
Getts, D. R. et al. Microparticles bearing encephalitogenic peptides induce T-cell tolerance and ameliorate experimental autoimmune encephalomyelitis. Nat. Biotech. 30, 1217–1224 (2012).
Kinman, L. et al. Lipid-drug association enhanced HIV-1 protease inhibitor indinavir localization in lymphoid tissues and viral load reduction: a proof of concept study in HIV-2287-infected macaques. J. Acquir. Immune Defic. Syndr. 34, 387–397 (2003).
Freeling, J. P., Koehn, J., Shu, C., Sun, J. & Ho, R. J. Y. Long-acting three-drug combination anti-HIV nanoparticles enhance drug exposure in primate plasma and cells within lymph nodes and blood. AIDS 28, 2625–2627 (2014).
Freeling, J. P. & Ho, R. J. Y. Anti-HIV drug particles may overcome lymphatic drug insufficiency and associated HIV persistence. Proc. Natl Acad. Sci. USA 111, E2512–E2513 (2014).
das Neves, J., Amiji, M. M., Bahia, M. F. & Sarmento, B. Nanotechnology-based systems for the treatment and prevention of HIV/AIDS. Adv. Drug Deliv. Rev. 62, 458–477 (2010).
Edagwa, B. J., Zhou, T., McMillan, J. M., Liu, X. M. & Gendelman, H. E. Development of HIV reservoir targeted long acting nanoformulated antiretroviral therapies. Curr. Med. Chem. 21, 4186–4198 (2014).
Sosnik, A., Chiappetta, D. A. & Carcaboso, Á. M. Drug delivery systems in HIV pharmacotherapy: what has been done and the challenges standing ahead. J. Control. Release 138, 2–15 (2009).
Lalanne, M. et al. Synthesis and biological evaluation of two glycerolipidic prodrugs of didanosine for direct lymphatic delivery against HIV. Bioorg. Med. Chem. Lett. 17, 2237–2240 (2007).
Skanji, R. et al. A new nanomedicine based on didanosine glycerolipidic prodrug enhances the long term accumulation of drug in a HIV sanctuary. Int. J. Pharm. 414, 285–297 (2011).
Puligujja, P. et al. Pharmacodynamics of long-acting folic acid-receptor targeted ritonavir-boosted atazanavir nanoformulations. Biomaterials 41, 141–150 (2015).
Horst, H. J. et al. Lymphatic absorption and metabolism of orally administered testosterone undecanoate in man. Klin. Wochenschr. 54, 875–879 (1976). One of the very few studies to have quantified drug uptake into the lymph in humans.
Edwards, G. A., Porter, C. J., Caliph, S. M., Khoo, S. M. & Charman, W. N. Animal models for the study of intestinal lymphatic drug transport. Adv. Drug Deliv. Rev. 50, 45–60 (2001).
Seeger, M. & Bewig, B. Ultrasound imaging of the thoracic duct. N. Engl. J. Med. 359, e28 (2008).
Nadolski, G. & Itkin, M. Thoracic duct embolization for the management of chylothoraces. Curr. Opin. Pulm. Med. 19, 380–386 (2013).
Thomas, S. N. & Schudel, A. Overcoming transport barriers for interstitial-, lymphatic-, and lymph node-targeted drug delivery. Curr. Opin. Chem. Engineer. 7, 65–74 (2015).
Miteva, D. O. Transmural flow modulates cell and fluid transport functions of lymphatic endothelium. Circ. Res. 106, 920–931 (2010).
Banerji, S. et al. LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan. J. Cell Biol. 144, 789–801 (1999).
Dixon, J. B., Raghunathan, S. & Swartz, M. A. A tissue-engineered model of the intestinal lacteal for evaluating lipid transport by lymphatics. Biotechnol. Bioengineer. 103, 1224–1235 (2009).
John, T. A., Vogel, S. M., Tiruppathi, C., Malik, A. B. & Minshall, R. D. Quantitative analysis of albumin uptake and transport in the rat microvessel endothelial monolayer. Am. J. Physiol. Lung Cell. Mol. Physiol. 284, L187–L196 (2003).
Schubert, W. et al. Caveolae-deficient endothelial cells show defects in the uptake and transport of albumin in vivo. J. Biol. Chem. 276, 48619–48622 (2001).
Mendelsohn, A. R. & Larrick, J. W. Sleep facilitates clearance of metabolites from the brain: glymphatic function in aging and neurodegenerative diseases. Rejuven. Res. 16, 518–523 (2013).
Thrane, V. R. et al. Paravascular microcirculation facilitates rapid lipid transport and astrocyte signaling in the brain. Sci. Rep. 3, 2582 (2013).
Florence, A. T. & Hussain, N. Transcytosis of nanoparticle and dendrimer delivery systems: evolving vistas. Adv. Drug Deliv. Rev. 50 (Suppl. 1), 69–89 (2001).
des Rieux, A., Fievez, V., Garinot, M., Schneider, Y. J. & Preat, V. Nanoparticles as potential oral delivery systems of proteins and vaccines: A mechanistic approach. J. Control. Release 116, 1–27 (2006).
Hunter, A. C., Elsom, J., Wibroe, P. P. & Moghimi, S. M. Polymeric particulate technologies for oral drug delivery and targeting: a pathophysiological perspective. Nanomedicine 8, S5–S20 (2012).
Ravi, P. R., Aditya, N., Kathuria, H., Malekar, S. & Vats, R. Lipid nanoparticles for oral delivery of raloxifene: optimization, stability, in vivo evaluation and uptake mechanism. Eur. J. Pharm. Biopharm. 87, 114–124 (2014).
Sun, M. et al. Intestinal absorption and intestinal lymphatic transport of sirolimus from self-microemulsifying drug delivery systems assessed using the single-pass intestinal perfusion (SPIP) technique and a chylomicron flow blocking approach: Linear correlation with oral bioavailabilities in rats. Eur. J. Pharm. Sci. 43, 132–140 (2011).
Zhang, Z. et al. Bile salts enhance the intestinal absorption of lipophilic drug loaded lipid nanocarriers: mechanism and effect in rats. Int. J. Pharm. 452, 374–381 (2013).
Fu, C. et al. The absorption, distribution, excretion and toxicity of mesoporous silica nanoparticles in mice following different exposure routes. Biomaterials 34, 2565–2575 (2013).
Dahan, A. & Hoffman, A. Evaluation of a chylomicron flow blocking approach to investigate the intestinal lymphatic transport of lipophilic drugs. Eur. J. Pharm. Sci. 24, 381–388 (2005).
Bernard, A., Carlier, H. & Caselli, C. Biochemical and ultrastructural study of actidione-cycloheximide effect on fat intestinal absorption in the rat (author's transl). J. Physiol. (Paris). 76, 147–157 (1980) (in French).
Alitalo, A. & Detmar, M. Interaction of tumor cells and lymphatic vessels in cancer progression. Oncogene 31, 4499–4508 (2012).
Hwee, Y. L. et al. Hypercholesterolemic mice exhibit lymphatic vessel dysfunction and degeneration. Am. J. Pathol. 175, 1328–1337 (2009).
Liao, S. et al. Impaired lymphatic contraction associated with immunosuppression. Proc. Natl Acad. Sci. USA 108, 18784–18789 (2011).
Bagby, T. R. et al. Lymphatic trafficking kinetics and near-infrared imaging using star polymer architectures with controlled anionic character. Eur. J. Pharm. Sci. 47, 287–294 (2012).
Karlsson, M. et al. “Tolerosomes” are produced by intestinal epithelial cells. Eur. J. Immunol. 31, 2892–2900 (2001).
Menard, S., Cerf-Bensussan, N. & Heyman, M. Multiple facets of intestinal permeability and epithelial handling of dietary antigens. Mucosal Immunol. 3, 247–259 (2010).
Wang, Y. H. et al. Chylomicrons promote intestinal absorption and systemic dissemination of dietary antigen (ovalbumin) in mice. PLoS ONE 4, e8442 (2009).
Jang, M. H. et al. Intestinal villous M cells: an antigen entry site in the mucosal epithelium. Proc. Natl Acad. Sci. USA 101, 6110–6115 (2004).
Neutra, M. R. & Kraehenbuhl, J. P. in Mucosal Immunology 3rd edn (eds Mestecky, J. et al.) 111–130 (Elsevier, 2005).
Caliph, S. M. et al. The impact of lymphatic transport on the systemic disposition of lipophilic drugs. J. Pharm. Sci. 102, 2395–2408 (2013).
Carrasco, Y. R. & Batista, F. D. B cells acquire particulate antigen in a macrophage-rich area at the boundary between the follicle and the subcapsular sinus of the lymph node. Immunity 27, 160–171 (2007).
Junt, T. Subcapsular sinus macrophages in lymph nodes clear lymph-borne viruses and present them to antiviral B cells. Nature 450, 110–114 (2007).
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