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1D0-520 - V5 CIW Site Designer - Dump Information

Vendor : CIW
Exam Code : 1D0-520
Exam Name : V5 CIW Site Designer
Questions and Answers : 294 Q & A
Updated On : April 19, 2019
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1D0-520 Questions and Answers

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1D0-520 V5 CIW Site Designer

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1D0-520 exam Dumps Source : V5 CIW Site Designer

Test Code : 1D0-520
Test Name : V5 CIW Site Designer
Vendor Name : CIW
Q&A : 294 Real Questions

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CIW V5 CIW Site Designer

licensed internet Webmaster | Real Questions and Pass4sure dumps

The certified information superhighway Webmaster has gained momentum over the last six months. All grasp CIW titles start with the CIW Foundations exam (1D-410). Passing this examination earns you the title of CIW associate. by the way, in case you passed the CompTIA I-internet+ exam, that you may follow the CompTIA certification towards the Foundations examination.

After the Foundations exam, candidates can plot their course to CIW master Administrator, enterprise Developer, dressmaker, or internet website supervisor. The certification title you pursue will assess how many checks you will should take to earn your title.

once a candidate passes the CIW Foundations exam and one of the crucial CIW job position exams, the particular person is a CIW knowledgeable. The CIW expert title sticks until the candidate passes all the vital tests to develop into a master CIW.

when you have information superhighway journey in any taste, this certification may well be a good selection so you might add to your repertoire.

CIW training requirements and career information | Real Questions and Pass4sure dumps

a certified web Webmaster requires some formal education. be taught about the connected schooling, career assistance and salary statistics to peer if this is the correct career for you.

certified internet site owners are IT professionals who design, build and maintain web sites. whereas there are minimal necessities, this certification requires candidates to enroll in one or extra tutorial courses and flow an examination.

simple counsel

licensed web site owners (CIW) are information technology (IT) gurus that design, construct and keep web sites. while certification is not vital to become a web developer, CIW is an expert standard identified via business, govt and academic associations. There are 4 programs obtainable in CIW, and college students can comprehensive these courses online or through CIW-sanctioned institutions.

Required training Completion of a CIW training software other requirementsCertification examination Projected Job boom (2014-2024)* eight% (network and computing device programs directors) Median Annual profits (2015)* $seventy seven,810 (network and laptop programs administrators)

source: *U.S. Bureau of Labor information

find faculties that offer these normal programs

  • counsel systems safety
  • Networking administration
  • techniques Administration
  • Webmaster and Multimedia management
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    The licensed web Webmaster (CIW) software changed into developed in the late Nineties through a gaggle of IT gurus as a means to supply and hold business requirements. nowadays, CIW training classes can be found on-line through the certain website ( they're also available at CIW-sanctioned tutorial associations, reminiscent of excessive faculties, neighborhood faculties and universities. There aren't any tutorial must haves mandatory to enter a CIW software.

    There are 4 CIW training courses: internet dressmaker, IT Administrator, internet web page supervisor and enterprise Developer. These courses are available at three certification tiers: affiliate, grasp and expert. associate and master classes each focus on one education software, while the skilled program encompasses examine in all 4 schooling courses.

    All CIW college students are required to take foundational courses in cyber web business, website building and network technology. particular person software coursework varies and can include examine in internet safety, server administration, database development, site design and Java programming. All classes culminate with a certification examination, which may also be taken at a CIW checking out middle. There are numerous trying out centers global.

    career suggestions

    CIW specialists have applications in a wide latitude of fields, including IT safety, e-commerce design, network server administration, systems administration, utility building and methods evaluation.

    in line with the U.S. Bureau of Labor records (BLS), job increase in these areas is anticipated to enhance between 2014 and 2024. The projected boom rate was 8% for network and laptop techniques directors, and 11% for database administrators.

    In 2015, the BLS suggested that the median annual income for community and computing device methods directors changed into $77,810, $forty eight,620 for computer consumer guide specialists and $131,600 for desktop and systems managers ( besides the fact that children, it's crucial to notice that the BLS facts did not differentiate between CIWs and non-licensed IT professionals.

    CIWs should be well versed in programming, internet design and other net connected considerations equivalent to web safety and databases. professional certification can support legitimatize credentials.

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    Huawei quietly launches the P30 Lite, starts taking pre-orders | real questions and Pass4sure dumps

    Huawei has quietly launched the P30 Lite after the launch of its premium variants, P30 and P30 Pro. The company has started taking pre-orders of the Lite version in the Philippines and Canada through its official website and third-party vendors as well.

    Like other Lite variants of their premium versions from Huawei, the P30 Lite has watered-down specifications of the P30. In terms of design, the P30 Lite is the same as its premium siblings. It features a 6.15-inch Full HD+ IPS LCD panel with a resolution of 1,080 x 2,340 pixels. The screen has a water-drop notch and very thin bezels on the sides, offering an immersive viewing experience.

    Under the hood is Huawei’s custom HiSilicon Kirin 710 SoC with Mali- G51 for graphics. It is coupled with 6 GB RAM and 128 GB internal storage, expandable up to 256 GB.

    For optics, the phone has a triple-camera setup. It includes a 24MP primary shooter with an aperture of f/1.8. The secondary lens has an 8MP ultra-wide sensor and 2MP depth sensor for bokeh. For selfies, the phone has a 32MP f/2.0 sensor with fixed focus. The camera includes features like Night mode, Portrait mode, Pro Mode, HDR, Time-lapse, etc.

    The P30 Lite packs a 3,340 mAh battery and has fast-charging support. However, it features support for an 18W charger compared to 22.5W on the P30.

    There is no face unlock, and the device has a fingerprint sensor at the back. Connectivity options include 4G VoLTE, Wi-Fi 802.11, Mobile Hotspot, Bluetooth v5.0, USB Type-C, and A-GPS. It runs on Android 9.0 with EMUI 9.0 on top. The P30 Lite would be available in three colour options — Midnight Black, Peacock Blue, and Pearl White and is priced at 16,990 Phillipine Pesos (approximately Rs 22,534 at current prices). 

    A proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine | real questions and Pass4sure dumps

    Design, expression and purification of 5x, 2x and 1xL2-VLP

    The RG1 epitope of HPV16 (QLYKTCKQAGTCPPDIIPKVEG), HPV16 and 18 (QLYKTCKQAGTCPPDIIPKVEGgsDLYKTCKQSGTCPPDVVPKVEG) or HPV16, 18, 35, 31 and 52 (QLYKTCKQAGTCPPDIIPKVEGgsDLYKTCKQSGTCPPDVVPKVEGgsQLYRTCKAAGTCPPDVIPKVEGgsQLYQTCKAAGTCPSDVIPKIEHgsQLYQTCKASGTCPPDVIPKVEG) was attached to the 3′ of the full-length SpyCatcher-AP205 gene27 by PCR. A small Glycine-Serine (GS) linker was inserted between L2 peptides followed by a Glycine-Glycine-Serine-Glycine (GGSG) linker between the SpyCatcher-VLPs and the L2 peptide(s). To create 1xL2-VLPs, the SpyCatcher-AP205 VLP served as template for the initial PCR, where GGSG-16L2 was added by including the nucleotide sequence for the L2 RG1 epitope of HPV16 in the reverse primer overhang.

    The 5x and 2xL2-VLPs were created by PCR. The SpyCatcher-AP205 VLP sequence27 was amplified with a 5′ NcoI site and a 3′ overlap capable of annealing to the 5′ of a HPV16 RG1 sequence. In the second PCR reaction, a synthetic gene construct (GeneArt, Thermo Scientific) containing the concatenated L2 RG1 epitope of HPV16, 18, 35, 31, 52 was used to create an amplicon with a 5′ overlap, capable of annealing to the 3′ end of the SpyCatcher-AP205 sequence and containing a 3′ NotI cloning site. For 5xL2-VLP, the full sequence was amplified, whereas only the 16 and 18L2 sequences were amplified for creating the 2xL2-VLPs. In a third PCR reaction, the amplicons of the first and second PCR were mixed in order to amplify the 5x and 2xL2-VLP constructs.

    The resulting 5xL2, 2xL2 and 1xL2 VLP genes were cloned into a pET-15b vector using NcoI and NotI restriction sites and the SpyCatcher-AP205-L2-pET-15b plasmid was transformed into E. coli One Shot® BL21 Star™ (DE3) cells (Thermo Scientific). The non-optimized small-scale upstream expression and downstream purification accomplished using Optiprep™ (Sigma) step gradients, as previously described27,28.

    Design, expression and purification of SpyTag-VAR2CSA-HIS antigen

    PCR added SpyTag (AHIVMVDAYKPTK) and a flexible GGS-linker 5′, as well as a V5 and hexa-histidine tag (HIS) 3′ to the gene coding for the DBL1-ID2A region (aa 9–1025) of VAR2CSA (FCR3 genotype)21 (herein termed VAR2CSA). The SpyTag-VAR2CSA-HIS gene sequence was subcloned into the pET-15b vector as described above, expressed in C3029H SHuffle T7 competent E.coli cells (New England Biolabs Inc.) and purified by IMAC and size exclusion chromatography (HiLoad Superdex 200 pg, GE Healthcare).

    Quality assessment of 5x, 2x, and 1x-L2-VLPs

    The 5x, 2x and 1x-L2-VLPs were assessed using negative stain transmission electron microscopy (TEM) as well as dynamic light scattering (DLS) as previously described27,28. Briefly, for TEM, the 5x, 2x and 1xL2-VLPs were adsorbed to 200-mesh carbon-coated grids. Grids were stained with 2% uranyl acetate (pH7.0) and analyzed with an accelerating voltage of 80 kV, using a CM 100 BioTWIN electron microscope (Phillips, Amsterdam).

    For DLS measurements, the distribution of particle sizes was acquired (658 nm, at 25 °C, WYATT Technology, DynaPro NanoStar). The 5x and 2xL2-VLPs were analyzed with 20 runs and the 1xL2-VLP with 10 runs. The estimated diameter of the main particle population and the percent polydispersity (%Pd), was calculated for all three L2-VLPs.

    Conjugation of VAR2CSA to 5x and 2xL2-VLPs

    To generate the combinatorial HPV and PM VLP vaccines, purified SpyTag-VAR2CSA-His and 2x or 5xL2-VLPs were mixed in a 3:1 molar ratio (VAR2CSA:VLP) and incubated for 16 h at 4 °C in a phosphate-buffered saline (PBS), pH 8 and adjusted to 0.6 M NaCl.

    Excess, unbound antigen was removed by dialysing (1000 kDa molecular weight cut-off, Spectrum Labs) against PBS pH8, 0.6 M NaCl.

    Vaccine formulation

    VLP vaccines (5xL2, 2xL2, 1xL2-VLP or the equivalent combinatorial vaccines) were formulated 1 hour prior to immunizations using 2%Alhydrogel (Brenntag) to a final concentration of 2 mg/ml aluminum hydroxide.

    Mouse immunization Studies

    8 week old, female BALB/c mice (Taconic, Denmark) were immunized intramuscularly (thigh muscle) with 3.14 µg antigen dose 1xL2 VLPs (based on the total L2-VLP amount in a prime-boost-boost regimen on week 1, 4 and 7 (Fig. 1d lower panel, n = 7). Blood samples were collected at week 9 and the in vitro HPV PsV challenge was performed on this serum. For the 5x and 2xL2-VLP vaccines (with and without VAR2CSA), 8 week old, female BALB/c mice (Jackson Laboratories, USA) were immunized with 5 µg (based on the total L2-VLP amount). Mice receiving the 5xL2 (n = 25) or 2xL2-VLP (n = 25) vaccines or the corresponding combinatorial vaccines (n = 6 in each group) were immunized in a prime-boost regimen on week 1 and 4 (Fig. 1d, upper panel). Blood samples were collected at week 5 and at week 14 (for 5xL2-VLP-Var2 and 2xL2-VLP-Var2) and week 20 (for 5 × -L2-VLP and 2xL2-VLP) to analyze HPV/VAR2CSA specific serum IgG titers and to perform the in vitro parasite binding-inhibition assay.

    Serum immunoglobulin levels

    Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of anti-VAR2CSA and anti-L2 immunoglobulin (IgG) levels. For VAR2CSA specific IgG titers, 96-well microtiter plates (Nunc MaxiSorp) were coated with 0.1 µg/well recombinant VAR2CSA protein (no SpyTag). The L2 peptide (aa 17–31) of the 16, 18, 31, 35, 45 or 51 HPV types was genetically fused to the N-terminus of an unrelated mycobacterium tuberculosis antigen (accession number WP_057363222, aa 44–338). For 5xL2- and 2xL2-VLP studies, the recombinant fusion proteins were used as coat protein for all the ELISAs. For the 1xL2-VLP study, ELISA plates were coated with synthetic L2 peptides (aa 17–31) of the HPV16, 18 or 31, through conjugation of the biotinylated peptides to streptavidin as described elsewhere18.

    Plates were developed with TMB and signal was measured at O.D.450. An O.D. cutoff 0.1 of was used to determine the endpoint titers of PM and HPV specific serum IgG.

    Parasite binding-inhibition assay

    An in vitro parasite binding-inhibition assay was employed to determine the capacity of antibodies induced by the 5x, and 2x-VLP-VAR2CSA vaccine to inhibit the binding between native VAR2CSA expressed on iE and CSA (decorin Sigma). For a detailed description of the assay see32,33. In short, panning of late-stage Plasmodium falciparum (homologous FCR3 or heterologous NF54 and 7G8 genotypes) parasites selected for a CSA-binding phenotype. CSA-binding parasites were labeled with Tritium and added to CSA-coated wells. Diluted, pooled VAR2CSA immune serum (5xL2-Var2 n = 6, 2xL2-Var2 n = 6 and 0xL2-Var2 n = 5) was added in triplicates. Unbound iE were washed off by a pipetting robot (Beckman-Coulter) and remaining iE were collected onto a filter plate (Perkin-Elmer). Liquid scintillation counting (Topcount NXT, Perkin-Elmer) determined the number of iE not inhibited by the VAR2CSA immune sera. Each assay measured mean iE binding and the percentage of binding iE was calculated by dividing the test result with the mean value of iE in wells incubated without serum.

    The assay was repeated three times for FCR3 and 7G8 and twice for NF54. The data points represent the mean percentage parasite binding across the technical repetitions of the assay. Error bars represent the standard deviation of this mean. BSA coated wells and naïve sera were used as a negative control.

    Pseudovirion Production

    Type 16, 31, 35, 45 and 52 HPV PsV containing a reporter plasmid encoding for both GFP and luciferase were generated using HEK293TT cells as described elsewhere34.

    The infectious unit (IU) count was determined by infecting HEK293TT cells for 48 h and measuring the percentage of infected (GFP-positive) cells by flow cytometry.

    In vitro L2 based HPV pseudovirion neutralization assay

    The capacity of anti-L2 antibodies to inhibit infection by the homologous HPV16 PsV as well as heterologous HPV45 PsV was determined for sera obtained from mice with 1xL2-VLP vaccine using a previously described L2-based HPV PsV neutralization assay35.

    In vivo HPV Pseudovirion Neutralization Assay

    Vaccine induced protection against HPV infection was evaluated in an in vivo PsV genital challenge model, as described previously17,18. Briefly, Balb/C mice previously immunized with 5xL2-, 2xL2-VLP, or the corresponding combinatorial vaccines, were randomized into challenge groups (n = 5 for 5xL2-VLP and 2xL2-VLP or n = 6 for 5xL2-VLP-Var2, 2xL2-VLP-Var2) starting approximately 3 weeks after the final boost. Five days prior to challenge, mice received 3 mg progesterone. Mice were briefly anesthetized using isoflurane and vaginally challenged with 6.3*106 infectious units (IU) PsV16, 3.15*107 IU PsV31, 8.84*106 IU PsV 35, 1.19*107 IU PsV45 or 6.2*105 IU PsV52. PsV infection was assessed under inhalation anesthesia (isoflurane) 48 hours after challenge by instilling 0.4 mg luciferin vaginally and measuring radiance (five minute exposure) using a Caliper IVIS Lumina II (Caliper Life Sciences). Background radiance was subtracted from this radiance value in order to determine average radiance (p/s/cm2/sr). Immunized mice were compared to groups of unvaccinated mice (n = 6 for PsV16 challenge and n = 3 for PsV31, 35, 45, and 52).

    Ethical Statement

    All animal studies were performed in accordance with Federation of European Laboratory Animal Science Associations (FELASA) and American Association for Laboratory Animal Science (AALAS) guidelines and regulations and were approved by the Danish Animal Experiments Inspectorate (Approval number: 2013-15-2934-00902/BES) and the University of New Mexico Institutional Animal Care and Use Committee (IACUC), Animal Welfare Assurance # D16–00228 (A3350-01) USDA Registration # 85-R-0014, Protocol No. 16-200498-HSC.

    Statistical Analysis

    GraphPadPrism7.0 was used for calculating geometric mean titers, geometric standard deviation, preparing all graphs and statistical calculations. The non-parametric, two tailed, Mann-Whitney Rank Sum Test was applied in order to determine statistical significance (P < 0.05).

    Black Shark 2 might be the best gaming phone yet | real questions and Pass4sure dumps

    Black Shark 2 might be the best gaming phone yet


    19 Mar 2019: Black Shark 2 might be the best gaming phone yet

    Xiaomi-backed Black Shark has launched its latest gaming smartphone in China today.

    Dubbed as Black Shark 2, the phone comes with a pressure-sensitive AMOLED display, dedicated liquid cooling technology, a 48MP dual rear-camera and top-end internals including Snapdragon 855 chipset.

    In China, the phone comes at a starting price of 3,199 yuan which is roughly Rs. 32,700 in India.

    Here's everything to know.

    Design & Display: Black Shark 2: At a glance

    Up front, the Black Shark 2 gets a regular, notch-less display with prominent bezels whereas the rear side gets dual cameras and a backlit company logo.

    It also features a dedicated Liquid Cool 3.0 technology for enhanced heat dissipation.

    Further, there's a 6.39-inch full-HD+ AMOLED display with 240Hz touch response rate and Magic Press technology that lets you configure pressure sensitivity on the display.

    Camera: What's the camera like on Black Shark 2?

    On the back, the Black Shark 2 gets a dual camera setup comprising a 48MP primary sensor with an f/1.75 aperture and a 12MP secondary sensor with an f/2.2 aperture. The main camera also offers a 2x optical zoom.

    Up front, the phone has a 20MP selfie camera with an f/2.0 aperture.

    Internals: Under the hood

    The Black Shark 2 is powered by a Qualcomm Snapdragon 855 chipset, paired with Adreno 640 GPU, upto 12GB of RAM and 128GB/256GB of internal storage.

    It also offers 4,000mAh battery, stereo speakers, dual-SIM support, 4G VoLTE, Wi-Fi 802.11a/c, Bluetooth v5.0, dual-band GPS, and USB Type-C port.

    Further, the phone packs all standard sensors including the in-trend in-display fingerprint sensor.

    Key details: Black Shark 2: Pricing and availability

    Black Shark 2 costs CNY 3,199 (Rs. 32,700) for the base-end 6GB RAM/128GB storage model. The mid-end 8GB/128GB and 8GB/256GB variants are priced at CNY 3,499 (Rs. 35,800) and CNY 3,799 (Rs. 38,800), respectively.

    The top-end 12GB/256GB model costs CNY 4,199 (Rs. 42,900).

    The pre-orders for the Black Shark 2 are currently open in China via the official Black Shark website, TMall, and

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